MEMBRANE BOUND RNA VIRUS REPLICATION COMPLEXES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 由正链RNA病毒复制的RNA普遍与膜和膜重排有关，如单膜或双膜小泡。然而，大多数情况下，相对于这些重排的膜，RNA合成的位置(S)以及膜、囊泡内部、腔室和细胞质之间的拓扑关系并不清楚。最近的结果表明，至少在某些情况下，病毒RNA复制发生在这样的小泡内。然而，这些发现留下了许多悬而未决的问题，在许多方面，这是由于所使用的二维分析所固有的局限性。鸟巢病毒(FHV)是诺达病毒家族中研究最多的成员，它在感染果蝇细胞的线粒体膜外膜上复制，在膜间隙形成膜结合的球状结构，类似于许多正链RNA病毒观察到的多泡结构。我们使用电子显微镜断层扫描(EMT)来解析FHV球状膜的形态。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA replication by positive-strand RNA viruses is universally associated with membranes and membrane rearrangements such as single or double membrane vesicles. However, in most cases the site(s) of RNA synthesis relative to these rearranged membranes and the topological relationships between membranes, vesicle interiors, lumenal compartments and the cytoplasm are poorly defined. Recent results suggest that, in at least some cases, viral RNA replication occurs inside such vesicles. However, these findings leave many unresolved questions, in many ways due to limitations inherent in the two-dimensional analyses used. The best-studied member of the nodavirus family, flock house virus (FHV), replicates at the outer mitochondrial membranes of infected Drosophila cells where membrane-bound spherules form in the intermembrane space, similar to multi-vesicular structures observed for many positive-strand RNA viruses. We employ electron microscope tomography (EMT) to resolve the morphology of the FHV spherule membranes.