MEMBRANE BOUND RNA VIRUS REPLICATION COMPLEXES

膜结合 RNA 病毒复制复合物

• 批准号: 7601028
• 负责人: PAUL G. AHLQUIST
• 金额: $ 1.3万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601028
• 关键词: Binding; Cell membrane; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytoplasm; DNA Sequence Rearrangement; Drosophila genus; Electron Microscope; Family; Funding; Grant; Housing; Institution; Left; Membrane; Morphology; Outer Mitochondrial Membrane; RNA Viruses; RNA chemical synthesis; RNA replication; Relative (related person); Research; Research Personnel; Resources; Site; Source; Structure; United States National Institutes of Health; Vesicle; Viral; Virus; Virus Replication; member; tomography; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA replication by positive-strand RNA viruses is universally associated with membranes and membrane rearrangements such as single or double membrane vesicles. However, in most cases the site(s) of RNA synthesis relative to these rearranged membranes and the topological relationships between membranes, vesicle interiors, lumenal compartments and the cytoplasm are poorly defined. Recent results suggest that, in at least some cases, viral RNA replication occurs inside such vesicles. However, these findings leave many unresolved questions, in many ways due to limitations inherent in the two-dimensional analyses used. The best-studied member of the nodavirus family, flock house virus (FHV), replicates at the outer mitochondrial membranes of infected Drosophila cells where membrane-bound spherules form in the intermembrane space, similar to multi-vesicular structures observed for many positive-strand RNA viruses. We employ electron microscope tomography (EMT) to resolve the morphology of the FHV spherule membranes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 正链RNA病毒的RNA复制普遍与膜和膜重排有关，例如单膜或双膜囊泡。 然而，在大多数情况下，相对于这些重排膜的 RNA 合成位点以及膜、囊泡内部、腔室和细胞质之间的拓扑关系尚不清楚。 最近的结果表明，至少在某些情况下，病毒 RNA 复制发生在此类囊泡内。 然而，由于所使用的二维分析固有的局限性，这些发现在很多方面留下了许多未解决的问题。 诺达病毒家族中研究最充分的成员羊群病毒 (FHV) 在受感染果蝇细胞的线粒体外膜上进行复制，在膜间隙中形成膜结合小球，类似于在许多正链 RNA 病毒中观察到的多囊泡结构。我们采用电子显微镜断层扫描 (EMT) 来解析 FHV 球膜的形态。