MEMBRANE BOUND RNA VIRUS REPLICATION COMPLEXES

膜结合 RNA 病毒复制复合物

• 批准号: 7601683
• 负责人: PAUL G. AHLQUIST
• 金额: $ 0.18万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601683
• 关键词: Alphavirus; Binding; Brome mosaic virus; Cells; Cellular Structures; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA Sequence Rearrangement; Dimensions; Drosophila genus; Electron Microscope; Endoplasmic Reticulum; Family Picornaviridae; Flavivirus; Funding; Grant; Housing; Imagery; Institution; Intracellular Membranes; Membrane; Outer Mitochondrial Membrane; RNA Viruses; RNA chemical synthesis; RNA replication; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; Vesicle; Viral; Virus; Virus Replication; mitochondrial membrane; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The use of electron microscope tomography revealed the membrane structure and topology of nodavirus RNA replication compartments. A universal feature of positive-strand RNA viruses is the involvement of host intracellular membranes in RNA replication. Intracellular membrane rearrangements associated with viral RNA replication have been observed for many positive-strand RNA viruses including alphaviruses, picornaviruses, flaviviruses, and others. In the nodavirus flock house virus (FHV), viral RNA synthesis occurs on mitochondrial membranes in association with formation of 40  60 nm vesicles or spherules between the inner and outer mitochondrial membranes. For brome mosaic virus in the alphavirus superfamily, RNA replication has been found to occur inside strikingly similar spherules on endoplasmic reticulum membranes. To better define the structure and topology of such viral RNA replication structures, we studied the spherules formed in FHV-infected Drosophila cells by electron microscope tomography, which permits the visualization of cellular structures in three dimensions.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 电子显微镜断层扫描的使用揭示了肿瘤病毒RNA复制室的膜结构和拓扑结构。阳性RNA病毒的普遍特征是宿主细胞内膜的参与RNA复制。对于许多阳性链RNA病毒，包括α病毒，picornaviruses，Flaviviviruse等许多阳性RNA病毒，已经观察到与病毒RNA复制相关的细胞内膜重排。在诺达病毒羊群病毒（FHV）中，病毒RNA合成发生在线粒体膜上，与内部和外部线粒体外膜之间形成40 60 nm囊泡或球形。对于Brome Mosaic病毒在α-病毒超家族中，已经发现RNA复制发生在内质网膜上非常相似的球形内。为了更好地定义这种病毒RNA复制结构的结构和拓扑结构，我们研究了通过电子显微镜断层扫描术在FHV感染的果蝇细胞中形成的球形，这允许在三个维度中可视化细胞结构。