MEMBRANE BOUND RNA VIRUS REPLICATION COMPLEXES

膜结合 RNA 病毒复制复合物

• 批准号: 7601683
• 负责人: PAUL G. AHLQUIST
• 金额: $ 0.18万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601683
• 关键词: Alphavirus; Binding; Brome mosaic virus; Cells; Cellular Structures; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA Sequence Rearrangement; Dimensions; Drosophila genus; Electron Microscope; Endoplasmic Reticulum; Family Picornaviridae; Flavivirus; Funding; Grant; Housing; Imagery; Institution; Intracellular Membranes; Membrane; Outer Mitochondrial Membrane; RNA Viruses; RNA chemical synthesis; RNA replication; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health; Vesicle; Viral; Virus; Virus Replication; mitochondrial membrane; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The use of electron microscope tomography revealed the membrane structure and topology of nodavirus RNA replication compartments. A universal feature of positive-strand RNA viruses is the involvement of host intracellular membranes in RNA replication. Intracellular membrane rearrangements associated with viral RNA replication have been observed for many positive-strand RNA viruses including alphaviruses, picornaviruses, flaviviruses, and others. In the nodavirus flock house virus (FHV), viral RNA synthesis occurs on mitochondrial membranes in association with formation of 40  60 nm vesicles or spherules between the inner and outer mitochondrial membranes. For brome mosaic virus in the alphavirus superfamily, RNA replication has been found to occur inside strikingly similar spherules on endoplasmic reticulum membranes. To better define the structure and topology of such viral RNA replication structures, we studied the spherules formed in FHV-infected Drosophila cells by electron microscope tomography, which permits the visualization of cellular structures in three dimensions.

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