MEMBRANE BOUND RNA VIRUS REPLICATION COMPLEXES

膜结合 RNA 病毒复制复合物

• 批准号: 7181368
• 负责人: PAUL G. AHLQUIST
• 金额: $ 0.43万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7181368
• 关键词: bioimaging /biomedical imaging; biomedical resource; image processing; technology /technique development; transmission electron microscopy

DESCRIPTION: The National Center for Microscopy and Imaging Research (NCMIR) was established to develop computer aided advanced microscopy for acqusition of structural and functional data in the dimensional range of 1 nm3 to 100 um3. With novel specimen staining methods, imaging instruments and computational capabilities, researchers are addressing the next great biological challenges in the post-genomic age by situating proteins and macromolecular complexes in their cellular and tissue environements. Resource instruments include intermediate high voltage transmission electron microscopes (IVEMs) and high-speed large-format laser-scanning light microscopes specially modified for "meso-scale" biological microscopy. Our core technological research is directed towards developing technologies that will contribute to structural neurobiology, cell and molecular biology. With NCMIR's participation in the Biomedical Informatics Research Network (BIRN), we are scaling up our ability to image at high resolution across wide expanses of brain tissue to reveal structural and molecular in mouse models of human disease. Our collaboration, service, training and dissemination programs expand the use of these technologies to maximize their value to the biomedical community. Core biological projects include development of new staining methods for IVEM and for correlated light and IVEM analyses of specimens to examine the architecture of neuronal systems and the dynamics of subcellular processes. Core research is tightly linked to collaborative projects, in areas such as animal of neurodegenerative diseases, stroke, heart disease, cancer, infectious diseases and diabetes. The capabilites and research accomplishments anticipated in specimen preparation are augmented by continued development of instruments and image processing facilities. We emphasize the use of computer-aided methods for enhancing image contrast and reconstructing larger structural complexes using 3D tomographic and serial thick section analysis. Extraction of 3D information on a large scale will be accelerated through the development of high-throughput methods for tomographic reconstruction, analysis and visualization. We continue to provide researchers greater access to the Resource through development of web-based remote control systems for the IVEM's and interfaces for computational grids and distributed databases via high-speed networks. We will expand outreach efforts via web-based tutorials, software and microscopic data repositories linked to other biological databases.

描述：国家显微镜和成像研究中心(NCMIR)的成立是为了开发计算机辅助高级显微镜，以获取1 Nm~3到100 mm~3的结构和功能数据。利用新的标本染色方法、成像仪器和计算能力，研究人员正在通过将蛋白质和大分子复合体放置在细胞和组织环境中来应对后基因组时代的下一个重大生物学挑战。资源仪器包括中高压传输电子显微镜(IVEM)和专门为“中尺度”生物显微镜改装的高速大幅面激光扫描光学显微镜。我们的核心技术研究旨在开发有助于结构神经生物学、细胞和分子生物学的技术。随着NCMIR参与生物医学信息学研究网络(BIRN)，我们正在扩大我们在大范围脑组织中进行高分辨率成像的能力，以揭示小鼠的结构和分子 人类疾病的模型。我们的合作、服务、培训和传播计划扩大了对这些技术的使用，以最大限度地提高它们对生物医学界的价值。核心生物学项目包括为IVEM和相关光开发新的染色方法 以及对样本进行IVEM分析，以检查神经元系统的结构和亚细胞过程的动力学。核心研究与合作项目紧密相连，涉及神经退行性疾病、中风、心脏病、癌症、传染病和糖尿病等领域。随着仪器和图像处理设施的不断发展，样品制备方面预期的能力和研究成果将得到加强。我们强调使用计算机辅助方法来增强图像对比度，并使用3D层析和连续厚切片分析来重建更大的结构复合体。通过开发用于层析重建、分析和可视化的高通量方法，将加速大规模提取3D信息。我们继续通过为IVEM开发基于网络的远程控制系统，以及通过高速网络为计算网格和分布式数据库开发接口，为研究人员提供更多访问资源的机会。我们将通过基于网络的教程、软件和链接到其他生物数据库的微观数据储存库来扩大推广工作。