CALCIUM ABSORPTION IN NEONATES FED WITH MOTHER'S MILK FORTIFIED WITH A DONOR

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 母乳被认为是婴儿的营养选择，包括新生儿重症监护室(NICU)中的早产儿。在极低出生体重(VLBW)婴儿的情况下，这种牛奶通常添加人乳强化剂(HMF)，以提供这些婴儿所需的额外营养。我们建议调查由巴氏杀菌的供者母乳制成的HMF，特别是关于钙的吸收、生长、骨矿状态、身体成分和喂养耐受性。我们假设这些研究参数将导致使用以供体母乳为基础的HMF的适当结果。 使用由巴氏杀菌的供体母乳制成的母乳强化剂将为早产儿提供足够的钙吸收。此外，这种新的强化剂将提供足够的生长和骨骼矿化。 具体目的：主要目的：测定用巴氏杀菌的供体母乳强化的母乳喂养的早产儿的钙吸收。 第二目的：(1)评价喂养巴氏杀菌供体母乳强化母乳的早产儿的生长发育、骨矿状况和身体成分；(2)评价喂养巴氏杀菌供体母乳强化母乳的早产儿喂养耐受性(试点数据)。 背景和意义：早产儿和小于2.0公斤的婴儿有独特的营养需求，包括在体重基础上需要比足月婴儿更高的蛋白质和矿物质摄入量。常规的以牛奶为基础的配方奶以及母亲自己的牛奶不足以满足这些要求。因此，几乎所有2.0公斤的婴儿都会被喂食自己的母乳，其中添加了以牛奶为基础的婴儿强化剂，或者，如果母亲没有证明她的牛奶量足够或选择不母乳喂养，他们将获得专为早产儿设计的基于牛奶的配方奶。虽然这些产品已经使用多年，并被证明是相对安全和有效的，但人们一直担心，接受牛奶喂养产品的早产儿感染和坏死性小肠结肠炎(NEC)的风险增加。已经证明，在接受母乳喂养的婴儿中，喂养耐受性增强，NEC风险降低，尽管不使用所有牛奶蛋白的具体好处尚不确定。 安全有效地避免使用所有以牛奶为基础的产品的可能性最近已经成为可能，这要归功于一种由巴氏杀菌的供体母乳制成的以人奶为基础的强化剂(Prolact-plus，Prolacta Bioscience，加利福尼亚州蒙罗维亚)。这种强化剂在预防感染和NEC方面的有效性将在2007年的一项多中心试验中进行测试。到目前为止，它只在个别病例和较小的生长试验中使用。然而，使用这种较新的捐赠者乳制品的生物等效性尚不清楚。具体来说，对早产儿至关重要的钙的适当水平和生物利用度尚未从Prolact-Plus等捐赠者乳制品中进行测试。因此，我们正在进行这项研究，以确定该产品的钙和骨矿效应，使用的方案类似于即将进行的多中心疗效试验。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Breast milk is considered the nutrition of choice for infants, including premature infants in the Neonatal ICU (NICU). In the case of very low birth weight (VLBW) infants, this milk is generally supplemented with a human milk fortifier (HMF) in order to provide the additional nutrients that these babies require. We propose to investigate a HMF made from pasteurized donor human milk, in particular regarding calcium absorption, growth, bone mineral status, body composition, and feeding tolerance. We hypothesize that these study parameters will result in adequate outcomes with the use of the donor milk based HMF. The use of a human milk fortifier made from pasteurized donor human milk will provide adequate calcium absorption to preterm infants. In addition, this new fortifier will provide adequate growth and bone mineralization. SPECIFIC AIMS: Primary Aim: To determine calcium absorption in premature infants fed mother's own milk fortified with pasteurized donor human milk fortifier. Secondary Aims: (1) To evaluate the growth, bone mineral status, and body composition of premature infants fed mother's own milk fortified with pasteurized donor human milk fortifier, and, (2) To assess feeding tolerance (pilot data) in infants fed mother's own milk fortified with pasteurized donor human milk. BACKGROUND AND SIGNIFICANCE:Infants who are premature and below about 2.0 kg have unique nutritional requirements including a need for higher intakes of protein and minerals on a body weight basis than full-term infants. Routine cow's milk based formula as well as mother's own milk are insufficient to meet those requirements. Therefore, virtually all infants < 2.0 kg are fed either their own mother's milk fortified with a cow's milk based powdered infant fortifier or, if the mother does not proved an adequate volume of her milk or chooses not to breast feed, they will receive a cow's milk based formula designed for premature infants. Although these products have been used for many years and have been shown to be relatively safe and effective, persistent concerns exist regarding an increased risk of infections and necrotizing enterocolitis (NEC) in premature infants who receive cow's milk based feeding products. It has been shown that feeding tolerance is enhanced and NEC risk is decreased in infants receiving human milk, although the specific benefits of avoiding all cow's milk protein are uncertain. The possibility of safely and effectively avoiding the use of all cow's milk based products has recently become possible due to the development of a human milk-based fortifier made from pasteurized donor human milk (Prolact-plus, Prolacta Bioscience, Monrovia, CA). The effectiveness of this fortifier in preventing infections and NEC will be tested in a multi-center trial in 2007. To date, it has been used only in individual cases and in smaller growth trials. However, the biological equivalence of using this newer donor milk product is not known. Specifically, the appropriate level and bioavailability of calcium, which is critical for preterm infants has not been tested from a donor milk product such as the Prolact-Plus. Therefore, we are conducting this study to determine the calcium and bone mineral effects of this product, using a protocol similar to that which will be used in the upcoming multi-center efficacy trial.