NMR ANALYSIS OF FOUR SAMPLES

四个样品的核磁共振分析

• 批准号: 7957544
• 负责人: Parastoo Azadi
• 金额: $ 0.22万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957544
• 关键词: Carbon; Chemicals; Computer Retrieval of Information on Scientific Projects Database; Coupling; Deuterium; Dimensions; Freeze Drying; Funding; Grant; Institution; Measures; NMR Spectroscopy; Protons; Relative (related person); Research; Research Personnel; Resources; Sampling; Scanning; Source; Time; United States National Institutes of Health; Width; quantum

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. NMR Spectroscopy The sample (70 mg) was deuterium-exchanged by lyophilization from D2O, dissolved in 700 uL D2O (99.996 % D). Heteronuclear single quantum coherence (gHSQC) spectra were acquired on a Varian Inova-500 MHz spectrometer at 313 K (40 ¿C). Proton chemical shifts were measured relative to DSS (delta=0.00 ppm). The spectra were recorded with a spectral width in the proton dimension of 2382 Hz and in the carbon dimension of 8750 Hz. The acquisition time was 0.20 s, and 100 increments were collected with 32 scans each. The one-bond C-H coupling constant was set at 140 Hz.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 NMR光谱 样品（70 mg）是通过D2O的冻干化脱发的，溶解在700 ul D2O（99.996％d）中。异核单量子相干性（GHSQC）光谱在313 K（40€）的Varian Inova-500 MHz光谱仪上获取。相对于DSS（DELTA = 0.00 ppm）测量质子化学位移。在2382 Hz的质子尺寸和8750 Hz的碳尺寸中记录了光谱宽度。采集时间为0.20 s，收集100个增量，每次扫描32次。一键C-H耦合常数设置为140 Hz。