NMR ANALYSIS OF ONE SAMPLE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 核磁共振波谱 样品(70 Mg)从D2O中冷冻干燥换氢，溶解在700ul D2O(99.996%D)中。在瓦里安Inova-500 MHz光谱仪上获得了313K(40℃)下的梯度增强异核单量子相干(GHSQC)谱。测量了相对于DSS(增量=0.00ppm)的质子化学位移。这些光谱的质子维度为3259赫兹，碳维度为12569赫兹。采集时间为0.2秒S，每次扫描采集到128个增量。单键C-H耦合常数设置为140赫兹。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. NMR Spectroscopy The sample (70 mg) was deuterium-exchanged by lyophilization from D2O, dissolved in 700 uL D2O (99.996 % D). A gradient enhanced heteronuclear single quantum coherence (gHSQC) spectrum was acquired on a Varian Inova-500 MHz spectrometer at 313 K (40 ¿C). Proton chemical shifts were measured relative to DSS (delta=0.00 ppm). The spectra were recorded with a spectral width in the proton dimension of 3259 Hz and in the carbon dimension of 12569 Hz. The acquisition time was 0.20 s, and 128 increments were collected with 64 scans each. The one-bond C-H coupling constant was set at 140 Hz.