ADVANCED OPTICAL MICROSCOPY IMAGING TECHNIQUES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 量子点和金纳米粒子由于其独特的光学性质，在医学上具有创新的诊断和治疗方法。我们正在将QDot和金纳米粒子注射到新鲜的动物组织中。与未注射的图像相比，胶原、血管和肌肉的双光子和倍频图像的质量和分辨率得到了显著的增强。特别是，我们已经能够看到不同水平的组织，这些组织在没有注射量子点的情况下仍然不可见。 在这个项目中，我们的目标是探索以可控的方式将量子点和金纳米粒子输送到单个细胞和组织内的替代策略。金纳米粒子由于其表面等离子体共振增强的光散射和吸收，在癌症诊断和治疗方面具有巨大的潜力。然后，这些金纳米粒子可以增加高达10^10倍的拉曼光谱信号。我们的主要目标是获得在生物事件中分离或浸入组织中的单个细胞的细胞内化学成分(振动键)的指纹，例如，DNA损伤/修复、应激、光消融、光修复、机械应力等。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Due to their unique optical properties Quantum Dots (QDs) and gold nanoparticles (NPs) promise innovative diagnostic and therapeutic methods in medicine. We are injecting QDots and gold NPs into fresh animal tissue. A remarkable enhancement on the quality and resolution of two-photons and SHG images of collagen, vessels, and muscle has been obtained in comparison with the images without being injected. In particular, we have been able to see different levels of tissue which remain invisible without the injection of QDs. In this project, we aim to explore alternative strategies for delivery in a controllable way QDs and gold NPs into a single cell and also inside tissue. Gold NPs have immense potential for cancer diagnostic and therapy on account of their surface plasmon resonance enhanced light scattering and absorption. These Gold NPs can then increase up to 10^10 times the Raman spectroscopy signal. Our main goal is to obtain the finger print of the intracellular chemical components (vibrational bonds) of a single cell isolated or immersed into tissue during biological events as for example, DNA damage/repair, stress, photoablation, photoporation, mechano-stress among others.