WOUND MIGRATION FORCE MONITORING

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为了研究细胞有丝分裂过程中活细胞的牵引力传递模式，我们将牵引力显微镜技术应用于微管蛋白转基因的PtK2细胞。我们将利用光学技术，包括光钳和激光刀片，以及分子生物学技术，包括药物抑制，来阐明微管在有丝分裂阶段从贴壁细胞到底物的牵引力传递中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To study the traction force transmission pattern of live cell during the cell mitosis, we will apply the traction force microscopy technique on the tubulin transfected PtK2 cell. We will use the optical techniques, including optical tweezer and laser blade and molecular biological techniques, including pharmacological inhibition to elucidate the roles of microtubules in the traction force transmission from the adherent cell to the substrate in the mitosis stage.