WOUND MIGRATION FORCE MONITORING

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 为了研究活细胞在细胞有丝分裂过程中牵引力的传递模式，我们将应用牵引力显微镜技术对微管蛋白转染的PtK 2细胞进行观察。 我们将使用光学技术，包括光镊和激光刀和分子生物学技术，包括药理学抑制来阐明微管在有丝分裂阶段从粘附细胞到基底的牵引力传递中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To study the traction force transmission pattern of live cell during the cell mitosis, we will apply the traction force microscopy technique on the tubulin transfected PtK2 cell. We will use the optical techniques, including optical tweezer and laser blade and molecular biological techniques, including pharmacological inhibition to elucidate the roles of microtubules in the traction force transmission from the adherent cell to the substrate in the mitosis stage.