WOUND MIGRATION FORCE MONITORING

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 为了研究细胞有丝分裂过程中活细胞的牵引力传递模式，我们将牵引力显微镜技术应用于微管蛋白转基因的PtK2细胞。我们将利用光学技术，包括光钳和激光刀片，以及分子生物学技术，包括药物抑制，来阐明微管在有丝分裂阶段从贴壁细胞到底物的牵引力传递中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. To study the traction force transmission pattern of live cell during the cell mitosis, we will apply the traction force microscopy technique on the tubulin transfected PtK2 cell. We will use the optical techniques, including optical tweezer and laser blade and molecular biological techniques, including pharmacological inhibition to elucidate the roles of microtubules in the traction force transmission from the adherent cell to the substrate in the mitosis stage.