LEGINON SOFTWARE DISTRIBUTION AND SUPPORT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 Leginon软件是通过资源传播的主要软件包。Leginon软件控制显微镜和摄像系统，并负责显微镜操作员通常执行的一系列复杂任务。该系统通常可以在单个实验过程中以各种放大比例获取数千张图像，并且这些图像和与其相关的参数由关系数据库管理。理想情况下，Leginon作为分布式系统运行，通过网络与仪器、数据库和文件存储进行通信，所有这些都可能位于不同的计算机上。该系统需要在将运行Leginon的所有计算机上安装Python编程包和扩展程序，在支持数据库的计算机上安装MySQL数据库，在将在基于网络的查看器中显示图像的计算机上安装装有PHP的网络服务器。此外，Leginon应用程序需要仔细的校准和设置程序，以便高效和有效地工作。将这种复杂的系统转移到学术实验室，可能不包括高级计算机人员的支持，这是一个重大挑战。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Leginon software is the primary software package disseminated through the Resource. The Leginon software controls the microscope and camera system, and is responsible for a complex set of tasks normally performed by the microscope operator. The system can typically acquire many thousands of images at a variety of magnification scales over the course of a single experiment and these images and the parameters associated with them are managed by a relational database. Leginon ideally runs as a distributed system communicating over a network to the instruments, database and file storage all of which may be located on separate computers. The system requires installation of the Python programming package and extensions on all computers that will run Leginon, a MySQL database on a computer supporting the database, and a webserver with PHP on a computer that will display the images in the web based viewers. In addition the Leginon applications require careful calibrations and setup procedures in order to work efficiently and effectively. Transferring a complex system of this sort to academic labs, which might not include support from high level computer personnel, is a major challenge.