Purchase of Applied Biosystems QTRAP 5500 Mass Spectrometer

购买 Applied Biosystems QTRAP 5500 质谱仪

• 批准号: 7792991
• 负责人: ALMA L BURLINGAME
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2011-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792991
• 关键词: Acetylation; Advisory Committees; Alzheimer' s Disease; Biology; Chemicals; Chromatin; Cues; Disease; Docking; Environment; Epilepsy; Financial Support; Funding; Generations; Goals; HIV; Housing; Human Biology; Individual; Investigation; Maintenance; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Methylation; Mission; Modification; Molecular; Mono-S; N.I.H. Research Support; Peptide Hydrolases; Peptides; Performance; Phosphorylation; Play; Post-Translational Protein Processing; Principal Investigator; Productivity; Proteins; Regulation; Research; Research Personnel; Research Project Grants; Resources; Role; Schedule; Set protein; Signal Pathway; Site; Supervision; Training; Ubiquitination; United States National Institutes of Health; design; instrument; mass spectrometer; multiple reaction monitoring; programs; protein complex; protein function; public health relevance; response; stoichiometry

DESCRIPTION (provided by applicant): This proposal seeks funding for the purchase of a third generation AB/Sciex QTRAP 5500 mass spectrometer. This shared instrument will be used for reliable quantitation of specific posttranslationally modified peptides down to the attomole level - a task for which the QTRAP 5500 was explicitly designed and is uniquely suited. The overall major focus is on studies of the site-specific dynamics and modulation of protein function in response to a variety of cellular cues for this diverse group of biomedical investigations. Sets of covalently modified peptides will be selected for each major project for quantitative measurements by multiple reaction monitoring (MRM). Covalent addition of regulatory and docking moieties (phosphorylation, O-GlcNAcylation, and methylation (mono-, di- and tri-), acetylation and ubiquitination of lysyl e-amino functions, etc.) are enzymatically reversible, but the action of proteolytic enzymes produce irreversible cleavages of particular sets of proteins themselves. The instrument will be housed in the NIH NCRR-supported Mass spectrometry Resource Center at the new Mission Bay Campus at UCSF. This environment assures the availability and involvement of the scientific, technical and management expertise required to optimize its performance and research productivity. The primary theme of the major users focuses on studies of the dynamics of posttranslationally regulated proteins that underlies formation and modulation of signaling pathways and networks as well as the recruitment of protein complexes such as those that regulate chromatin biology. Investigators have major needs in measurements of individual site-specific stoichiometries and their temporal modulation by cellular cues. They are a group of six Principal Investigators involved in some twenty-one NIH supported research programs. These research projects are at the forefront of research in human biology and its disease states. Dr. Charles Craik will chair the Advisory Committee responsible for the overall guidance of the shared instrument's use. Mr. David Maltby will oversee optimization of performance, scheduling, training, maintenance and will provide daily supervision of usage. The close association with the UCSF-NCRR resource and the financial support of the major users will assure long-term productive usage. PUBLIC HEALTH RELEVANCE: Chemical modifications of proteins regulate their function. Impaired regulation of these modifications plays a role in such diseases as HIV, cancer, Alzheimer's disease and epilepsy. Our research goal is to reproducibly quantify levels of protein modification to gain an understanding of the molecular causes of the previously mentioned diseases.

描述（由申请人提供）：本提案寻求购买第三代AB/Sciex QTRAP 5500质谱仪的资金。该共享仪器将用于精确定量特定翻译后修饰肽至原子水平- QTRAP 5500明确设计的任务，并且是唯一适合的。总的来说，主要的重点是研究位点特异性动力学和蛋白质功能的调节，以响应各种细胞线索，用于这一多样化的生物医学研究。将为每个主要项目选择一组共价修饰肽，通过多反应监测（MRM）进行定量测量。调控和对接部分的共价加成（磷酸化、o- glcnac酰化和甲基化（单-、二-和三-）、赖氨酸e-氨基功能的乙酰化和泛素化等）在酶上是可逆的，但蛋白水解酶的作用对特定的蛋白质组本身产生不可逆的切割。该仪器将被安置在国立卫生研究院ncrr支持的质谱资源中心，该中心位于加州大学旧金山分校的新使命湾校区。这种环境确保了科学、技术和管理专业知识的可用性和参与，以优化其性能和研究生产力。主要用户的主要主题集中在翻译后调节蛋白质的动力学研究，这些蛋白质是信号通路和网络的形成和调节的基础，以及调节染色质生物学的蛋白质复合物的募集。研究人员主要需要测量个体位点特异性化学计量及其细胞线索的时间调节。他们是一个由六名首席研究员组成的小组，参与了大约21个NIH支持的研究项目。这些研究项目处于人类生物学及其疾病状态研究的前沿。Charles Craik博士将担任咨询委员会主席，负责对共享仪器的使用进行全面指导。David Maltby先生将监督性能优化、调度、培训、维护，并提供日常使用监督。与UCSF-NCRR资源的密切联系以及主要用户的财政支持将确保长期有效的使用。