Self Renewal and Differentiation of Embryonic Stem Cells

这个名为“人类胚胎干细胞的自我更新和分化”的应用程序由我们的人类胚胎干细胞核心实验室和4个项目组成。我们建议大幅扩大在我们目前资助的华盛顿大学/弗雷德·哈钦森癌症研究中心（UW/FHCRC）“人类胚胎干细胞研究探索中心”期间开始的工作。我们的人类胚胎干细胞（hESC）核心实验室，由Carol Ware领导，已经获得并表征了21个NIH批准的人类ESC系中的14个，并协助18个实验室进行与hESC相关的工作。核心将获得NIH批准的剩余hESC系，将表征所有系的microRNA表达谱，协助所有组成项目，并将继续协助将hESCs提供给当地80多个与hESCs相关的实验室。项目1由Blau和Ware领导，将研究由短链脂肪酸丁酸激活的自我更新的替代途径。项目2由Randall Moon领导，将研究规范和非规范Wnt信号

This application, entitled "Self Renewal and Differentiation of Human Embryonic Stem Cells," is comprised of our Human ES Cell Core Laboratory and 4 Projects. We propose to dramatically expand the work began during our current funding as a University of Washington/Fred Hutchinson Cancer Research Center (UW/FHCRC) "Exploratory Center for Human Embryonic Stem Ceil Research." Our Human ES Cell (hESC) Core Lab, directed by Carol Ware, has acquired and characterized 14 of the 21 NIH approved human ESC lines, and has assisted 18 laboratories in work related to hESCs. The Core will acquire the remaining NIH approved hESC lines, will characterize microRNA expression profiles of all lines, assist all of the component Projects, and will also continue to assist in making hESCs accessible to a local community of more than 80 labs with interests relevant to hESCs. Project 1, led by Blau and Ware, will examine an alternative pathway for self renewal that is activated by the short chain fatty acid, butyrate. Project 2, led by Randall Moon, will examine canonical and non-canonical Wnt signaling pathways in hESCs. Project 3, headed by Charles Murry, examines hESCs during differentiation directed toward a cardiomyocyte fate while Project 4, led by Tom Reh, evaluates directed differentiation toward a neuroretinal fate. Logical and extensive interactions between each of the Projects, and extensive interactions between the hESC Core Lab, each the Projects and other labs at UW/FHCRC create a Program vastly stronger than the sum of its parts.