Cell Biosystems Firefly 3000 system

Cell Biosystems Firefly 3000 系统

• 批准号: 7792826
• 负责人: C. Anthony Blau
• 金额: $ 31.84万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-15 至 2011-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7792826
• 关键词: Address; Cell Count; Cell Separation; Cells; Commit; Core Facility; Fees; Fireflies; Fred Hutchinson Cancer Research Center; Funding; Housing; Laboratories; Maintenance; Pediatric Hospitals; Population; Protein Analysis; Protein Isoforms; Research; Research Infrastructure; Sample Size; Sampling; Signal Transduction; Signaling Protein; System; Universities; Washington; instrument; interest; laser capture microdissection; operation; public health relevance

DESCRIPTION (provided by applicant): We request funds to support the purchase an instrument that will help more than 19 different laboratories at the University of Washington (UW), Seattle Children's Hospital, and the Fred Hutchinson Cancer Research Center (FHCRC) address critical barriers in their research. The Firefly 3000 Protein Analysis System (Cell Biosciences, Palo Alto, CA) allows all isoforms of a signaling protein of interest to be precisely quantified using sample sizes containing as few as 25 cells. The ability to quantitatively assess cell signaling using very low cell numbers opens the door to assessing signal transduction in rare cell populations obtained after cell sorting or after laser capture microdissection. This instrument will be housed in our Cell Analysis Core Facility at the UW's South Lake Union Campus, using the same infrastructure that supports operation of the Core. In addition, the UW has committed a total of $120,000 over 5 years to support the maintenance of the instrument, assuring that user fees can be kept at relatively low levels. PUBLIC HEALTH RELEVANCE: This application seeks funding for the Firefly 3000 Protein Analysis System. This instrument will support more than 19 labs at the UW, FHCRC and Seattle Children's Hospital, allowing signal transduction to be quantitatively assessed using samples of as few as 25 cells.

描述（由申请人提供）：我们请求资金支持购买一台仪器，该仪器将帮助华盛顿大学 (UW)、西雅图儿童医院和 Fred Hutchinson 癌症研究中心 (FHCRC) 的超过 19 个不同实验室解决其研究中的关键障碍。 Firefly 3000 蛋白质分析系统（Cell Biosciences，帕洛阿尔托，加利福尼亚州）允许使用包含少至 25 个细胞的样本量来精确定量感兴趣的信号蛋白的所有亚型。使用非常低的细胞数量定量评估细胞信号转导的能力为评估细胞分选或激光捕获显微切割后获得的稀有细胞群中的信号转导打开了大门。该仪器将安装在威斯康星大学南湖联合校区的细胞分析核心设施中，使用支持核心运行的相同基础设施。此外，华盛顿大学还承诺在 5 年内提供总计 12 万美元的资金来支持该仪器的维护，以确保用户费用能够保持在相对较低的水平。 公共健康相关性：本申请为 Firefly 3000 蛋白质分析系统寻求资金。该仪器将支持 UW、FHCRC 和西雅图儿童医院的超过 19 个实验室，允许使用少至 25 个细胞的样本对信号转导进行定量评估。