Cell Biosystems Firefly 3000 system

描述(由申请人提供)：我们申请资金支持购买一台仪器，该仪器将帮助华盛顿大学(UW)、西雅图儿童医院和弗雷德·哈钦森癌症研究中心(FHCRC)的19个不同实验室解决他们研究中的关键障碍。Firefly 3000蛋白质分析系统(加州帕洛阿尔托细胞生物科学公司)允许使用只包含25个细胞的样本大小来精确定量感兴趣的信号蛋白质的所有异构体。使用非常低的细胞数量定量评估细胞信号的能力为评估在细胞分选或激光捕获显微切割后获得的稀有细胞群中的信号转导打开了大门。该仪器将安装在我们位于华盛顿大学南湖联合校区的细胞分析核心设施中，使用支持核心操作的相同基础设施。此外，UW承诺在5年内总共提供120,000美元，以支持仪器的维护，确保用户费用可以保持在相对较低的水平。 公共卫生相关性：该应用程序寻求为Firefly 3000蛋白质分析系统提供资金。该仪器将支持华盛顿大学、FHCRC和西雅图儿童医院的19个以上实验室，允许使用最少25个细胞的样本对信号转导进行定量评估。

DESCRIPTION (provided by applicant): We request funds to support the purchase an instrument that will help more than 19 different laboratories at the University of Washington (UW), Seattle Children's Hospital, and the Fred Hutchinson Cancer Research Center (FHCRC) address critical barriers in their research. The Firefly 3000 Protein Analysis System (Cell Biosciences, Palo Alto, CA) allows all isoforms of a signaling protein of interest to be precisely quantified using sample sizes containing as few as 25 cells. The ability to quantitatively assess cell signaling using very low cell numbers opens the door to assessing signal transduction in rare cell populations obtained after cell sorting or after laser capture microdissection. This instrument will be housed in our Cell Analysis Core Facility at the UW's South Lake Union Campus, using the same infrastructure that supports operation of the Core. In addition, the UW has committed a total of $120,000 over 5 years to support the maintenance of the instrument, assuring that user fees can be kept at relatively low levels. PUBLIC HEALTH RELEVANCE: This application seeks funding for the Firefly 3000 Protein Analysis System. This instrument will support more than 19 labs at the UW, FHCRC and Seattle Children's Hospital, allowing signal transduction to be quantitatively assessed using samples of as few as 25 cells.