FATTY ACID ANALYSIS OF 2 SAMPLES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 采用气相色谱/质谱仪(GC/MS)联用技术对酸解产物中脂肪酸的甲基衍生物进行了糖基组成分析。 从样品中取出一份等分，加入不同的试管中。然后，干样在温和的酸处理后，在80℃下(16小时)，在1M的HCl中甲醇中进行甲醇分解，得到脂肪酸甲酯。脂肪酸甲酯的GC/MS分析在AT 6890N GC与5975B MSD连接，使用Supelco EC-1熔融石英毛细管柱(30m×0.25 mm ID)。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Glycosyl composition analysis was performed by combined gas chromatography/mass spectrometry (GC/MS) Methyl derivatives of the fatty acids produced from the sample by acidic methanolysis. An aliquot was taken from the sample and added to separate tubes. Fatty acid methyl esters were then prepared from the dry sample following the mild acid treatment by methanolysis in 1 M HCl in methanol at 80¿C (16 hours). GC/MS analysis of the Fatty acid methyl esters was performed on an AT 6890N GC interfaced to a 5975B MSD, using a Supelco EC-1 fused silica capillary column (30m ¿ 0.25 mm ID).