Trace Metals Core

微量金属核心

• 批准号: 8065871
• 负责人: Joseph H Graziano
• 金额: $ 40.39万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-21 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8065871
• 关键词: AA Spectrophotometry; Acids; Arsenic; Bangladesh; Basic Science; Biological; Biological Markers; Biomedical Research; Blood; Blood Donations; Blood specimen; Carbon; Cells; Child; Childhood; Clara cell; Cohort Studies; Coupled; Creatinine; Enzyme-Linked Immunosorbent Assay; Epidemiologic Studies; Exposure to; Grant; Health; High Pressure Liquid Chromatography; Intelligence; Laboratories; Lead; Manganese; Mass Spectrum Analysis; Measurement; Measures; Metabolism; Metal exposure; Metals; Methodology; National Institute of Environmental Health Sciences; Natural graphite; New Hampshire; Oxidative Stress; Performance; Plasma; Proteins; Quality Control; Reaction; Research Personnel; Research Project Grants; Running; Sample Size; Sampling; Serum; Site Visit; Specimen; Structure of nail of toe; Superfund; Technology; Testing; Toxic effect; Trace metal; Urine; analytical method; base; blood lead; cost; drinking water; exposed human population; indium arsenide; member; novel; programs; protein metabolite; tool; urinary

Biomarkers of exposure to metals and metalloids have proven to be powerful tools for epidemiologic studies that seek to study associations between exposures and human health effects. The Trace Metals Core Lab came into existence in 1994 as part of our NIEHS Center, and received a rating of outstanding at NIEHS Center site visits that occurred in 1997 and 2002. The Laboratory participates in multiple quality control programs and has an outstanding record of past performance. Biomedical research Projects #2, #3 and #4, which take place in Bangladesh and New Hampshire, propose to test a set of novel hypotheses concerning the mechanisms and effects of exposure to As and Mn from drinking water. These projects collectively require the analysis of more than 60,000 biological specimens; this estimate approximates the total of 70,000 specimens that have been successfully measured during the past five years. The biological specimens to be analyzed and stored in the Trace Metals Core Lab include blood, urine and toenails. The methodologies employed will include graphite furnace atomic absorption spectrophotometry (GFAAS) (e.g., arsenic in urine and lead in blood), inductively-coupled plasma mass spectrometry with dynamic reaction cell (ICP-MS-DRC) (e.g., total As, Mn and Se in blood), HPLC coupled to ICP-MS-DRC (e.g., arsenic metabolites in urine and blood), and ELISA technology (clara cell protein-16). The Trace Metals Core Laboratory is well equipped to carry out these and other requested analyses at no cost to members of this Superfund Basic Research Program.

暴露于金属和类金属的生物标志物已被证明是流行病学研究的有力工具 旨在研究暴露与人类健康影响之间的关联。微量金属核心实验室 1994年作为我们NIEHS中心的一部分成立，并在NIEHS获得了杰出的评级 1997年和2002年进行的中心现场访视。 实验室参与了多个质量控制计划，并在过去的质量控制方面有着出色的记录。 性能生物医学研究项目#2，#3和#4，发生在孟加拉国和新西兰 汉普郡，提出测试一套新的假设，有关机制和影响的接触， 饮用水中的砷和锰。这些项目总共需要分析60，000多个 生物标本;这一估计接近70，000标本，已成功 在过去的五年里， 待分析并储存在微量金属核心实验室的生物标本包括血液、尿液和 脚趾甲采用的方法包括石墨炉原子吸收分光光度法 （GFAAS）（例如，尿中的砷和血中的铅），电感耦合等离子体质谱法， 动态反应池（ICP-MS-DRC）（例如，血液中的总As、Mn和Se），HPLC与ICP-MS-DRC联用 (e.g.,尿和血液中的砷代谢物）和ELISA技术（Clara细胞蛋白-16）。微量 金属岩心实验室设备齐全，可以免费进行这些和其他要求的分析， 这个超级基金基础研究项目的成员