Trace Metals Core

微量金属核心

• 批准号: 8065871
• 负责人: Joseph H Graziano
• 金额: $ 40.39万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-21 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8065871
• 关键词: AA Spectrophotometry; Acids; Arsenic; Bangladesh; Basic Science; Biological; Biological Markers; Biomedical Research; Blood; Blood Donations; Blood specimen; Carbon; Cells; Child; Childhood; Clara cell; Cohort Studies; Coupled; Creatinine; Enzyme-Linked Immunosorbent Assay; Epidemiologic Studies; Exposure to; Grant; Health; High Pressure Liquid Chromatography; Intelligence; Laboratories; Lead; Manganese; Mass Spectrum Analysis; Measurement; Measures; Metabolism; Metal exposure; Metals; Methodology; National Institute of Environmental Health Sciences; Natural graphite; New Hampshire; Oxidative Stress; Performance; Plasma; Proteins; Quality Control; Reaction; Research Personnel; Research Project Grants; Running; Sample Size; Sampling; Serum; Site Visit; Specimen; Structure of nail of toe; Superfund; Technology; Testing; Toxic effect; Trace metal; Urine; analytical method; base; blood lead; cost; drinking water; exposed human population; indium arsenide; member; novel; programs; protein metabolite; tool; urinary

Biomarkers of exposure to metals and metalloids have proven to be powerful tools for epidemiologic studies that seek to study associations between exposures and human health effects. The Trace Metals Core Lab came into existence in 1994 as part of our NIEHS Center, and received a rating of outstanding at NIEHS Center site visits that occurred in 1997 and 2002. The Laboratory participates in multiple quality control programs and has an outstanding record of past performance. Biomedical research Projects #2, #3 and #4, which take place in Bangladesh and New Hampshire, propose to test a set of novel hypotheses concerning the mechanisms and effects of exposure to As and Mn from drinking water. These projects collectively require the analysis of more than 60,000 biological specimens; this estimate approximates the total of 70,000 specimens that have been successfully measured during the past five years. The biological specimens to be analyzed and stored in the Trace Metals Core Lab include blood, urine and toenails. The methodologies employed will include graphite furnace atomic absorption spectrophotometry (GFAAS) (e.g., arsenic in urine and lead in blood), inductively-coupled plasma mass spectrometry with dynamic reaction cell (ICP-MS-DRC) (e.g., total As, Mn and Se in blood), HPLC coupled to ICP-MS-DRC (e.g., arsenic metabolites in urine and blood), and ELISA technology (clara cell protein-16). The Trace Metals Core Laboratory is well equipped to carry out these and other requested analyses at no cost to members of this Superfund Basic Research Program.

事实证明，接触金属和金属的生物标志物是流行病学研究的强大工具 试图研究暴露与人类健康影响之间的关联。痕量金属核心实验室 作为我们NIEHS中心的一部分，1994年成立，并在Niehs获得了杰出评级 中心地点访问于1997年和2002年。 该实验室参与了多个质量控制计划，并且有出色的过去记录 表现。生物医学研究项目＃2，＃3和＃4，发生在孟加拉国和新的 汉普郡提议测试有关暴露于机制的机制和影响的一组新型假设 从饮用水中使用MN。这些项目集体需要分析超过60,000 生物标本；该估计约有70,000个标本已成功 在过去五年中测量。 要分析和存储在痕量金属核心实验室中的生物标本包括血液，尿液和 脚趾甲。所采用的方法将包括石墨炉原子吸收分光光度法 （GFAAS）（例如，尿液中的砷和血液中的铅），感应耦合的等离子体质谱法与 动态反应细胞（ICP-MS-DRC）（例如，血液中的总为Mn和SE），HPLC与ICP-MS-DRC耦合 （例如，尿液和血液中的砷代谢产物）和ELISA技术（Clara Cell Protein-16）。痕迹 金属核心实验室有足够的能力进行这些和其他要求的分析 这个超级基础研究计划的成员。