ISOTOPE-ASSISTED DIFFERENTIAL METABOLOMICS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 从拟南芥的100个重要代谢物的代表那里获得额外的光谱信息，并填充数据库。在未来，我们将有一个目录，其中包含在所有人体组织中含量最丰富的10,000或更多小分子的结构。当我们的身体不断变化时，我们还将能够使用这些信息来实时监测他们的浓度波动。这项提议的目的是让我们离创造新陈代谢技术更近一步，以填补这一空白。这项建议探索使用生物样品的稳定同位素标记，结合分析的高灵敏度的质谱仪(MS)和高场核磁共振光谱的化学选择性，作为高通量代谢物分析的使能技术。我们称这一新方法为同位素辅助代谢组学。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Obtain additional spectral information from representatives of the 100 important metabolites of Arabidopsis and populate the database. In the future, we will have a catalog containing the structures of the 10,000 or more' most-abundant small molecules in all human tissues. We will also be able to use this information to monitor their fluctuations in concentration in real time, as our bodies continually change. The purpose of this proposal is to take us one step closer to creating metabolomic technologies to fill this void. This proposal explores the use of stable isotope labeling of biological samples coupled with the analytical high sensitivity of mass spectrometry (MS) and the chemical selectivity of high-field NMR spectroscopy as enabling technologies for high-throughput analysis of metabolomes. We call this novel approach isotope assisted metabolomics.