SUBCOMPLEXES OF THE SPINDLE POLE BODY

主轴极体的子复合体

• 批准号: 8171455
• 负责人: Trisha N. Davis
• 金额: $ 1.25万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171455
• 关键词: Baculoviruses; Cells; Centrosome; Collaborations; Complex; Computer Retrieval of Information on Scientific Projects Database; Eukaryotic Cell; Funding; Grant; Institution; Microtubules; Nuclear; Organelles; Proteins; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Work; Yeasts; gamma Tubulin; interest; reconstitution; spindle pole body

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The yeast spindle pole body (SPB) is functionally equivalent to the centrosome in higher eukaryotic cells. The SPB is a multilayered organelle that organizes both the nuclear and cytoplasmic microtubules. We are working toward reconstitution of the microtubule nucleating activity of the SPB. The well characterized small gamma-tubulin complex contains three proteins: Tub4p, Spc97p, and Spc98p. We have expressed all three proteins in baculovirus and found that they readily assemble into a stable, soluble complex. However, the nucleation capacity of the small complex is quite low, suggesting we are missing components required for nucleation. We have recently found that gamma-tubulin and Spc97p are also present as part of larger complex in yeast cells. We are interested in characterizing this complex in collaboration with the Yeast Resource Center.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 酵母纺锤体极体（SPB）在功能上等同于高等植物中的中心体。 真核细胞SPB是一个多层的细胞器，它组织细胞核和 细胞质微管 我们正致力于微管的重建 SPB的成核活性。 特征良好的小γ-微管蛋白复合物 包含三种蛋白质：Tub 4p、Spc 97 p和Spc 98 p。我们已经表达了所有三种蛋白质 并发现它们容易组装成稳定可溶性复合物。然而，在这方面， 小络合物的成核能力相当低，这表明我们错过了 成核所需的组分。我们最近发现γ-微管蛋白和 spc 97 p也作为酵母细胞中较大复合物的一部分存在。 我们有兴趣 与酵母资源中心合作，描述了这个复杂的结构。