PARTICLE TRACKING - DYNAMICS OF NANO-SCALE PARTICLES IN REAL TIME

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 很难观察到纳米级(~50 nm)粒子在生命系统中的动力学。这是由于检测仪器使用的波长的限制。通过应用圆扫描算法和多光子激发显微镜，我们能够实时测量运动粒子的轨迹，提供重要的信息，如染色质动力学在相间。除了轨迹外，下一步我们想继续观察运动粒子的方向和形状的变化。这提供了有关粒子如何相互作用的进一步信息，以及生命系统中更详细的动态信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The dynamics of the particles of nano-scaled (~50nm) in the living system was hard to observed. That was due to the limitation of the wavelength used in the detection instruments. By applying the circular scan algorithm and the multi-photon excitation microscopy, we were able to measure the the moving particle trajectories in real time, providing important information such as the chromatin dynamics during interphase. Besides the trajectories, the next step we would like to move forward to observe the orientation and shape changes of the moving particles. This provides further message about how the particles interact and more detailed dynamic information in the living system.