PARTICLE TRACKING - DYNAMICS OF NANO-SCALE PARTICLES IN REAL TIME

粒子追踪 - 纳米级粒子的实时动力学

• 批准号: 8170969
• 负责人: ENRICO GRATTON
• 金额: $ 12.29万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170969
• 关键词: Algorithms; Chromatin; Computer Retrieval of Information on Scientific Projects Database; Detection; Funding; Grant; Institution; Interphase; Measures; Microscopy; Organism; Research; Research Personnel; Resources; Scanning; Shapes; Source; Time; United States National Institutes of Health; instrument; multi-photon; nanoscale; particle

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The dynamics of the particles of nano-scaled (~50nm) in the living system was hard to observed. That was due to the limitation of the wavelength used in the detection instruments. By applying the circular scan algorithm and the multi-photon excitation microscopy, we were able to measure the the moving particle trajectories in real time, providing important information such as the chromatin dynamics during interphase. Besides the trajectories, the next step we would like to move forward to observe the orientation and shape changes of the moving particles. This provides further message about how the particles interact and more detailed dynamic information in the living system.

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