PARTICLE TRACKING - DYNAMICS OF NANO-SCALE PARTICLES IN REAL TIME

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 纳米尺度（~ 50 nm）的粒子在生命系统中的动力学很难观察到。 这是由于检测仪器所用波长的限制。 通过应用圆扫描算法和多光子激发显微镜，我们能够测量运动粒子的轨迹在真实的时间，提供重要的信息，如染色质动力学在间期。 除了轨迹，下一步我们想向前移动，观察运动粒子的方向和形状变化。 这提供了关于粒子如何相互作用的进一步信息，以及生命系统中更详细的动态信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The dynamics of the particles of nano-scaled (~50nm) in the living system was hard to observed. That was due to the limitation of the wavelength used in the detection instruments. By applying the circular scan algorithm and the multi-photon excitation microscopy, we were able to measure the the moving particle trajectories in real time, providing important information such as the chromatin dynamics during interphase. Besides the trajectories, the next step we would like to move forward to observe the orientation and shape changes of the moving particles. This provides further message about how the particles interact and more detailed dynamic information in the living system.