MICROPROBE X-RAY FLUORESCENCE & X-RAY SPECTROSCOPY ON BIOLOGICAL SAMPLES

微型探针 X 射线荧光

• 批准号: 7722770
• 负责人: RAUL BARREA
• 金额: $ 1.9万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722770
• 关键词: Animal Model; Area; Biological; Cancerous; Computer Retrieval of Information on Scientific Projects Database; Educational workshop; Elements; Fluorescence; Funding; Grant; Hand; Institution; KLK3 gene; Laboratories; Maps; Measurement; Neutrons; Prostate; Research; Research Personnel; Resources; Roentgen Rays; Running; Sampling; Scanning; Schools; Silicon; Source; Spectrum Analysis; Spottings; Standards of Weights and Measures; Students; System; Techniques; Tissues; Training; United States National Institutes of Health; absorption; base; cost; detector; research study; size; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. X-ray fluorescence on biological samples was performed at the 18ID BioCAT microprobe as part of a hands-on workshop BioCAT ran as part of the National School on Neutron and X-ray Scattering, August 12-25, 2007, Argonne National Laboratory Normal and cancerous prostate tissue from animal models were studied by scanning a 5 micron size x-ray beam in order to determine the elemental composition, particularly Ca, Fe, Cu and Zn, of the samples as a two dimensional map. The 12 keV X-ray beam was focused by using a KB mirrors system. A single element silicon drift detector completed the setup. X-ray absorption spectroscopy measurements at the Cu K edge were also performed on the same samples on selected areas. Based on the elemental maps, selected sample spots were scanned to obtain XANES spectra at the Cu K edge. Comparison with Cu standards was also performed to demonstrate the ability of the technique to identify different Cu species. A group of 20 students attended the experiment at Biocat. We have found that participating in APS run workshops a very cost and effort-effective way to do training and dissemination.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在18 ID BioCAT微探针上对生物样品进行X射线荧光，作为BioCAT作为国家中子和X射线散射学校的一部分运行的实践研讨会的一部分，2007年8月12-25日，阿贡国家实验室通过扫描5微米尺寸的X射线束来研究来自动物模型的正常和癌性前列腺组织，以确定元素组成，特别是Ca，Fe、Cu和Zn的二维分布图。12 keV的X射线束通过使用KB反射镜系统聚焦。 单元素硅漂移探测器完成了设置。在Cu K边缘的X射线吸收光谱测量也进行了相同的样品上选定的区域。基于元素图，扫描选定的样品点，以获得在Cu K边缘的XANES光谱。还进行了与Cu标准品的比较，以证明该技术鉴别不同Cu物质的能力。一组20名学生参加了在Biocat的实验。我们发现，参加APS举办的研讨会是一种非常成本和努力有效的培训和传播方式。