MICROPROBE X-RAY FLUORESCENCE & X-RAY SPECTROSCOPY ON BIOLOGICAL SAMPLES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 生物样品的X射线荧光是在18ID BioCAT微型探头上进行的，这是作为国家中子和X射线散射学院的一部分的动手研讨会的一部分，2007年8月12-25日，阿贡国家实验室通过扫描5微米尺寸的X射线束研究了动物模型的正常和癌前列腺组织，以确定样品的元素组成，特别是钙、铁、铜和锌，作为二维图。利用KB反射镜系统对12keV的X射线束进行了聚焦。一个单元件硅漂移探测器完成了设置。同样的样品也在选定的区域进行了铜K边的X射线吸收光谱测量。在元素图的基础上，对选定的样品点进行扫描，获得了位于CuK边的XANES光谱。并与铜标准进行了比较，以验证该技术识别不同铜形态的能力。一组20名学生参加了Biocat的实验。我们发现，参加APS举办研讨会是一种非常划算的培训和传播方式。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. X-ray fluorescence on biological samples was performed at the 18ID BioCAT microprobe as part of a hands-on workshop BioCAT ran as part of the National School on Neutron and X-ray Scattering, August 12-25, 2007, Argonne National Laboratory Normal and cancerous prostate tissue from animal models were studied by scanning a 5 micron size x-ray beam in order to determine the elemental composition, particularly Ca, Fe, Cu and Zn, of the samples as a two dimensional map. The 12 keV X-ray beam was focused by using a KB mirrors system. A single element silicon drift detector completed the setup. X-ray absorption spectroscopy measurements at the Cu K edge were also performed on the same samples on selected areas. Based on the elemental maps, selected sample spots were scanned to obtain XANES spectra at the Cu K edge. Comparison with Cu standards was also performed to demonstrate the ability of the technique to identify different Cu species. A group of 20 students attended the experiment at Biocat. We have found that participating in APS run workshops a very cost and effort-effective way to do training and dissemination.