High-Throughput Structural Biology

描述(由申请人提供)：本提案请求支持将于2012年1月22-27日在科罗拉多州Keystone举行的题为高通量结构生物学的Keystone研讨会，该会议由Ian A.Wilson、Aed M.Edwards、Tracy M.Handel和Andrej Sali组织。结构生物学仍然是进行生物学和生物医学发现的最多产和最丰富的方法之一，并提供对生物系统的基本分子见解。基因组测序数量的迅速增长为结构生物学家提供了大量新的机会和挑战，以探索生物大分子的巨大多样性及其与功能、进化和疾病的关系。会议的目标是探索结构生物学的当前进展和前沿，并说明如何利用X射线结晶学和核磁共振中的高通量方法--结合使用计算和其他生物物理技术的混合方法(包括低温电子显微镜、X射线和电子断层摄影术、SAXS、质谱学、蛋白质组学)--来解决分子、细胞和化学生物学中的热门和具有挑战性的问题。本次研讨会将集中讨论结构生物学家和生物学家可用于研究生物系统及其组成的大分子和络合物的各种途径和方法。我们还将通过两次公开辩论讨论一些与该领域密切相关的热点问题。与会者还将有机会通过关于结构-功能-生物组织的姊妹会议扩大他们对复杂生物系统的认识，这些复杂的生物系统可以受益于结构生物学目前的这些进展--该会议将与本次会议举行一次主旨会议和两次全体会议。 与公共健康相关：结构基因组学领域包括确定蛋白质和其他大分子的三维结构和功能的尖端实验方法。这些技术使科学界能够跟上从基因组测序工作中获得的丰富信息的步伐。关于高通量结构生物学的Keystone研讨会会议将集中讨论如何解决具有挑战性的蛋白质、生物系统和整个生物体，以及如何填补我们对蛋白质宇宙的知识空白，这一空白已经从大量生物功能未知的基因组序列中显现出来。

DESCRIPTION (provided by applicant): This proposal requests support for a Keystone Symposia meeting entitled High-Throughput Structural Biology, organized by Ian A. Wilson, Aled M. Edwards, Tracy M. Handel and Andrej Sali, which will be held in Keystone, Colorado from January 22 - 27, 2012. Structural Biology continues to be one of the most prolific and informative ways to make biological and biomedical discoveries, and provides fundamental molecular insights into biological systems. The rapidly expanding numbers of sequenced genomes is providing structural biologists with a wealth of new opportunities and challenges to explore the enormous diversity of biological macromolecules and their relationships to function, evolution and disease. The meeting goal is to explore current advances and frontiers in structural biology and illustrate how high-throughput approaches in x-ray crystallography and NMR - in combination with hybrid methods using computational and other biophysical techniques (including CryoEM, Xray and Electron Tomography, SAXS, Mass Spectometry, Proteomics) - can be used to tackle topical and challenging problems in molecular, cellular and chemical biology. This symposium will focus on the variety of approaches and methodologies that are available to both structural biologists and biologists for investigation of biological systems and their component macromolecules and complexes. We will also address some topical issues of great relevance to the field through two open debates. The attendees will also have an opportunity to broaden their appreciation of the complex biological systems that can benefit from these current advances in structural biology via the sister meeting on Structure-Function- Biological Organization - which will share a keynote and two plenary sessions with this meeting. PUBLIC HEALTH RELEVANCE: The field of structural genomics encompasses cutting-edge experimental methods to determine the three- dimensional structure and function of proteins and other macromolecules. These technologies enable the scientific community to keep pace with the wealth of information derived from genome sequencing efforts. The Keystone Symposia meeting on High-Throughput Structural Biology will focus on how to address challenging proteins, biological systems and whole organisms, as well as how to fill the void in our knowledge of the protein universe that has become apparent from the vast numbers of genome sequences where the biological function is unknown.