High-Throughput Structural Biology

描述（由申请人提供）：本提案要求支持由Ian a . Wilson, Aled M. Edwards, Tracy M. Handel和Andrej Sali组织的题为“高通量结构生物学”的Keystone专题会议，该会议将于2012年1月22日至27日在科罗拉多州的Keystone举行。结构生物学仍然是生物学和生物医学发现的最多产和最翔实的方法之一，并为生物系统提供了基本的分子见解。基因组测序数量的迅速增加，为结构生物学家探索生物大分子的巨大多样性及其与功能、进化和疾病的关系提供了大量新的机遇和挑战。会议的目标是探索结构生物学的最新进展和前沿，并说明x射线晶体学和核磁共振的高通量方法-结合使用计算和其他生物物理技术（包括CryoEM， x射线和电子断层扫描，SAXS，质谱，蛋白质组学）的混合方法-可以用来解决分子，细胞和化学生物学中的局部和具有挑战性的问题。本次研讨会将集中讨论结构生物学家和生物学家用于研究生物系统及其组成大分子和复合物的各种方法和方法。我们还将通过两次公开辩论来讨论与该领域密切相关的一些专题问题。与会者还将有机会通过“结构-功能-生物组织”姊妹会议扩大他们对复杂生物系统的认识，这些系统可以从结构生物学的当前进展中受益，该会议将在本次会议上分享主题演讲和两次全体会议。

DESCRIPTION (provided by applicant): This proposal requests support for a Keystone Symposia meeting entitled High-Throughput Structural Biology, organized by Ian A. Wilson, Aled M. Edwards, Tracy M. Handel and Andrej Sali, which will be held in Keystone, Colorado from January 22 - 27, 2012. Structural Biology continues to be one of the most prolific and informative ways to make biological and biomedical discoveries, and provides fundamental molecular insights into biological systems. The rapidly expanding numbers of sequenced genomes is providing structural biologists with a wealth of new opportunities and challenges to explore the enormous diversity of biological macromolecules and their relationships to function, evolution and disease. The meeting goal is to explore current advances and frontiers in structural biology and illustrate how high-throughput approaches in x-ray crystallography and NMR - in combination with hybrid methods using computational and other biophysical techniques (including CryoEM, Xray and Electron Tomography, SAXS, Mass Spectometry, Proteomics) - can be used to tackle topical and challenging problems in molecular, cellular and chemical biology. This symposium will focus on the variety of approaches and methodologies that are available to both structural biologists and biologists for investigation of biological systems and their component macromolecules and complexes. We will also address some topical issues of great relevance to the field through two open debates. The attendees will also have an opportunity to broaden their appreciation of the complex biological systems that can benefit from these current advances in structural biology via the sister meeting on Structure-Function- Biological Organization - which will share a keynote and two plenary sessions with this meeting. PUBLIC HEALTH RELEVANCE: The field of structural genomics encompasses cutting-edge experimental methods to determine the three- dimensional structure and function of proteins and other macromolecules. These technologies enable the scientific community to keep pace with the wealth of information derived from genome sequencing efforts. The Keystone Symposia meeting on High-Throughput Structural Biology will focus on how to address challenging proteins, biological systems and whole organisms, as well as how to fill the void in our knowledge of the protein universe that has become apparent from the vast numbers of genome sequences where the biological function is unknown.