Request for live cell confocal microscope for BSL3 facility

请求为 BSL3 设施提供活细胞共聚焦显微镜

• 批准号: 7787740
• 负责人: SIMON C WATKINS
• 金额: $ 40.59万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7787740
• 关键词: Area; Cells; Communities; Confocal Microscopy; Containment; Devices; Electrons; Environment; Equipment; Funding; Grant; Growth; HIV; Housing; Image; Image Analysis; Institution; Laboratories; Life; Light; Medical; Medical Research; Methods; Microscope; Photons; Research; Research Personnel; Research Project Grants; Scanning; Services; Specimen; Universities; biosafety level 3 facility; cellular imaging; instrument; medical schools; operation; optical imaging; pathogen; time use

DESCRIPTION (provided by applicant): This request is for funds to purchase a Nikon Ti inverted microscope with widefield, DIC and confocal capabilities. This instrument will be housed within imaging core of the Regional Biocontainment Laboratory(RBL) at the University of Pittsburgh. This imaging core is a newly formed satellite facility of the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of the CBI is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. The building of an imaging core within the RBL is an essential facet of that mandate Confocal microscopy is an essential service provided by the center, and there are multiple point scanning single photon and one multiphoton microscope available to users, however these devices are within an open lab environment and can only be used with fixed or non-pathogenic specimens. In the last few years there has been a dramatic increase in the number of funded grants within our institution that need to image pathogenic specimens in real time using live cell imaging. This cannot be done at the moment and it is imperative we build a specialized imaging facility within a barrier facility. While some projects may be performed in a level 2+ facility (for example imaging HIV) there are an equal number of projects that demand a level 3 facility. Rather than build 2 satellites we have decided to build a single facility in level 3 containment space that will be used by all users who need access to live cell imaging at containment level 2+ or 3. Obtaining funds for a live cell confocal for this space is the focus of this application. It is important to recognize that the CBI has two applications in this competition for similar equipment. The other application, for a live cell confocal with TIRF capabilities will be placed in the 24 hr/day imaging core space, this microscope cannot be used by any of the researchers included in this proposal. Because of this there is absolutely no redundancy between the two applications. Since the CBI commenced operations 17 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 200 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The device requested here is a critical component of the continued growth of this institutional facility, such that it can serve the needs of researchers who want to integrate live cell imaging of pathogens into their research.

描述（由申请人提供）：本申请用于购买具有广角、DIC和共聚焦功能的尼康Ti倒置显微镜。该仪器将被安置在匹兹堡大学区域生物控制实验室（RBL）的成像核心内。该成像核心是匹兹堡大学医学院生物成像中心（CBI）新成立的卫星设施。CBI的任务是为匹兹堡大学医学院的所有研究小组提供全面的光和电子光学、图像分析和形态测量方法。在RBL内建立成像核心是该任务的一个重要方面，共聚焦显微镜是该中心提供的一项基本服务，用户可以使用多点扫描单光子显微镜和一台多光子显微镜，但是这些设备是在开放的实验室环境中，只能用于固定或非致病标本。在过去的几年里，在我们的机构中，需要使用活细胞成像实时成像病原标本的资助资助数量急剧增加。目前还无法做到这一点，我们必须在屏障设施内建立一个专门的成像设施。虽然有些项目可能在2+级设施中进行（例如艾滋病毒成像），但同样数量的项目需要3级设施。我们决定不建造2颗卫星，而是在3级密闭空间建造一个单独的设施，供所有需要在2+或3级密闭空间获得活细胞成像的用户使用。为这个空间获得活细胞共聚焦的资金是本申请的重点。重要的是要认识到，CBI在这次类似设备的竞争中有两个应用程序。另一个应用，对于具有TIRF功能的活细胞共聚焦，将被放置在24小时/天的成像核心空间中，该显微镜不能被本提案中的任何研究人员使用。因此，两个应用程序之间绝对没有冗余。自17年前CBI开始运作以来，它已成为医学研究界的一个组成部分，目前参与医疗领域内200多个公共卫生服务资助小组的研究项目，以及公共卫生服务支助的其他部门和邻近机构的研究人员的项目。这里要求的设备是这个机构设施持续发展的关键组成部分，这样它就可以满足那些想要将病原体的活细胞成像整合到他们的研究中的研究人员的需求。