Mass Spectrometry

提供了 质谱法已经成为鉴定和表征蛋白质和其他生物学特性的主要方法。 大分子结构是生物过程的功能机器。的目的 质谱和蛋白质组学设施（MSPF）是为癌症中心成员提供必要的 大分子结构分析服务。这些服务包括电喷雾和MALDI质量 质谱法用于分子量测定，串联质谱法（MSn）用于详细结构测定 分析（即，用于分析复杂生物混合物的LC/MS/MS分析，以及 蛋白质鉴定的数据库搜索。该设施有两个电喷雾离子阱（LC/MS）系统 （ThermoElectron LCQ和LTQ）和高性能MALDI qToF系统（PE/Sciex proOTof）。的 该设施是在收费服务和共享使用（研究人员运行自己的样本）的基础上运行的。 机构人员提供样品制备、分析和数据解释方面的专业知识。他们还 协助实验设计和开发新的方法。在最近12个月内， 在本报告期内，来自所有五个研究项目的19名癌症中心成员使用了CCMSPF。 同行评审的使用量占总使用量的72%。这一核心资源的年度预算为408，887美元，其中 69%是机构资金，11%是用户费用，20%（81，000美元）是CCSG的要求。

PROVIDED. Mass spectrometry has become the principal method to identify and characterize proteins and other macromolecular structures that are the functional machinery for biological processes. The purpose of the Mass Spectrometry & Proteomics Facility (MSPF) is to provide Cancer Center members with essential services in macromolecular structural analysis. These services include electrospray and MALDI mass spectrometry for molecular mass determination, tandem mass spectrometry (MSn) for detailed structure analysis (i.e., peptide sequencing), LC/MS/MS analysis for analyzing complex biological mixtures, and database searching for protein identification. The facility has two electrospray ion trap (LC/MS) systems (ThermoElectron LCQ and LTQ) and a high performance MALDI qToF system (PE/Sciex prOTof). The facility is operated both on a fee-for-service and shared use (researchers run their own samples) basis. Facility personnel provide expertise on sample preparation, analysis and data interpretation. They also assist experimental design and the development of novel methodologies. During the most recent 12-month reporting period, the CCMSPF was used by 19 Cancer Center members from all five Research Programs. Peer-reviewed usage represented 72% of total usage. Annual budget for this core is $408,887, of which 69% is institutional funding, 11% is user fees, and 20% ($81,000) is requested from the CCSG.