Mass Spectrometry

但前提是。 质谱学已经成为鉴定和表征蛋白质和其他物质的主要方法 作为生物过程的功能机械的大分子结构。该计划的目的是 质谱学和蛋白质组学设施(MSPF)为癌症中心成员提供必要的 服务于大分子结构分析。这些服务包括电喷雾和MALDI质量 用于分子质量测定的光谱，用于详细结构的串联质谱仪(MSN) 用于分析复杂生物混合物的LC/MS/MS分析(即多肽测序)以及 在数据库中搜索蛋白质鉴定。该设施有两个电喷雾离子捕集(LC/MS)系统 (ThermoElectron Lcq和LTQ)和高性能MALDI qToF系统(PE/Sciex prOTof)。这个 该设施既按服务收费，又以共享使用(研究人员自己运行样本)的方式运营。 设施人员提供样品准备、分析和数据解释方面的专业知识。他们也 协助实验设计和新方法的开发。在最近12个月内 在报告期间，来自所有五个研究项目的19名癌症中心成员使用了CCMSPF。 同行评审的使用量占总使用量的72%。这一核心的年度预算为408,887美元，其中 69%是机构资金，11%是用户费用，20%(81,000美元)是向CCSG申请的。

PROVIDED. Mass spectrometry has become the principal method to identify and characterize proteins and other macromolecular structures that are the functional machinery for biological processes. The purpose of the Mass Spectrometry & Proteomics Facility (MSPF) is to provide Cancer Center members with essential services in macromolecular structural analysis. These services include electrospray and MALDI mass spectrometry for molecular mass determination, tandem mass spectrometry (MSn) for detailed structure analysis (i.e., peptide sequencing), LC/MS/MS analysis for analyzing complex biological mixtures, and database searching for protein identification. The facility has two electrospray ion trap (LC/MS) systems (ThermoElectron LCQ and LTQ) and a high performance MALDI qToF system (PE/Sciex prOTof). The facility is operated both on a fee-for-service and shared use (researchers run their own samples) basis. Facility personnel provide expertise on sample preparation, analysis and data interpretation. They also assist experimental design and the development of novel methodologies. During the most recent 12-month reporting period, the CCMSPF was used by 19 Cancer Center members from all five Research Programs. Peer-reviewed usage represented 72% of total usage. Annual budget for this core is $408,887, of which 69% is institutional funding, 11% is user fees, and 20% ($81,000) is requested from the CCSG.