Cytogenetics

细胞遗传学核心设施提供细胞遗传学、分子细胞遗传学和分子克隆性 City of Hope Cancer Center（CC）的研究人员。该设施的主要服务包括：细胞系 完整性表征和验证、组织（细胞）培养、实体瘤细胞遗传学、 基因组不稳定性，荧光显微镜和荧光原位杂交（FISH）研究， 非整倍体检测、基因追踪、特异性基因改变、基因型/表型靶向FISH（T-FISH） 残留疾病检测分析和基因整合/定位研究。光谱核型分析或24- 彩色核型分析、小鼠模型系统的20色核型分析和FICTION（荧光 免疫表型和间期细胞遗传学作为研究肿瘤的工具）或T-FISH （表型分析，然后是基因型分析）。酪胺信号放大 当怀疑靶水平较低时，FISH用于核酸的细胞定位;例如，在 基因或过继免疫治疗研究。由于CC成员的需求，我们扩大了服务范围， 提供（1）克隆性检测，以监测克隆性造血，这是治疗相关急性 在接受剂量强化方案的一些患者中观察到髓性白血病/骨髓增生异常; （2）细菌人工克隆（BAG）阵列（aCGH）用于全面的分子核型分析。我们也 为希望筛选自己的研究或需要 广泛使用。最后，这个核心也是SWOG细胞遗传学的参考FISH核心实验室 程序.在最近12个月的报告期内，使用了细胞遗传学共享资源 来自所有5个研究项目的18名癌症中心成员。同行评审的使用率占97%， 全部使用。这一核心的年度预算为292 811美元，其中22%为机构供资，22%为用户供资。 费26%来自其他来源，29%来自CCSG。

The Cytogenetics Core Facility provides cytogenetics, molecular cytogenetics, and molecular clonality assays to City of Hope Cancer Center (CC) investigators. Primary services of this facility include: cell line characterization and verification of integrity, tissue (cell) culturtng, solid tumor cytogenetics, assays for genomic instability, fluorescence photomicroscopy, and fluorescence in situ hybridization (FISH) studies for aneuploidy detection, gene tracking, specific gene alterations, genotype/phenotype targeted FISH (T-FISH) analyses for residual disease testing, and gene integration/mapping studies. Spectral karyotyping or 24- color karyotyping, 20-color karyotyping for mouse model systems, and FICTION (fluorescence immunophenotyping and interphase cytogenetics as a tool for investigation of neoplasms) or T-FISH (sequential phenotypic followed by genotype analysis) are also available. Tyramide signal amplification FISH is used for the cellular localization of nucleic acids when target levels are suspected to be low; e.g., in gene or adoptive immunotherapy studies. Due to CC members' needs, we expanded our services to provide (1) clonality testing to monitor for clonal hematopoiesis, a precursor of therapy-related acute myelogenous leukemia/myelodysplasia observed in some patjents who received dose-intensive regimens; and (2) Bacterial Artificial Clones (BAG) arrays (aCGH) for comprehensive molecular karyotyping. We also provide imaging analysis training for scientists who wish to screen their own studies or who require extensive usage. Finally, this core is also the reference FISH core laboratory for the SWOG cytogenetics program. During the most recent 12-month reporting period, the Cytogenetics shared resource was used by 18 Cancer Center members from all 5 Research Programs. Peer-reviewed usage represented 97% of total usage. Annual budget for this core is $292,811, of which 22% is institutional funding, 22% is user fees. 26% is from other sources, and 29% is requested from the CCSG.