Functional Genomics

功能基因组学核心(FGC)的目标是提供一种全面、集成的方法来 癌症中心研究人员的基因组学研究。目前，FGC提供了一系列微阵列和 数据分析服务。微阵列基因表达谱服务基于Affymetrix GeneChips的寡核苷酸阵列平台。基本和高级数据分析由 生物统计学家和生物信息学家与生物医学信息学核心合作。FGC是 目前正在进行扩张，以提供两项新的主要服务，基于RNAi的技术和基因分型。 前者允许使用siRNA等方法对基因进行功能分析，而后者提供 中等吞吐量的SNP和DMA甲基化分析。所有FGC服务均由Highly提供 经验丰富的核心管理人员，在其专业领域具有丰富的专业知识。凝聚力和 所有FGC组成部分之间的协同作用由科学主任提供，他领导定期 与所有核心经理举行会议，讨论分析开发、数据分析、故障排除、集成、 和新技术。核心经理在实验设计之前向研究人员提供建议 研究和解释过程中的实验室和临床研究、分析开发和质量控制 核心生成的数据。这种“一站式购物”的方法使功能基因组学的研究 癌症中心的研究人员可以广泛访问。因此，FGC提供了先进的技术和 支持基础、临床和人口研究，以加强功能基因组学研究和 跨所有COHCCC计划的协作。在前12个月的报告期内，FGC是 由来自所有5个研究项目的14名癌症中心成员使用；同行评审的使用率为76% 在总数中。拟议的年度预算总额为339,788美元，占机构支助的39%，用户的18% 其他费用16%，向CCSG申请27%(91,000美元)。

The goal of the Functional Genomics Core (FGC) is to provide a comprehensive, integrated approach to genomics research for Cancer Center investigators. Presently, the FGC provides a range of Microarray and Data Analysis Services. The microarray gene expression profiling service is based on the Affymetrix oligonucleotide array platform of GeneChips. Basic and advanced data analysis is provided by biostatisticians and bioinformaticists in conjunction with the Biomedical Informatics Core. The FGC is currently undergoing expansion to provide two new major services, RNAi-based technology and genotyping. The former allows functional analyses of genes using approaches such as siRNA, and latter provides moderate-throughput SNP and DMA methylation analyses. All of the FGC services are provided by highly experienced Core Managers with extensive expertise in their areas of specialization. Cohesiveness and synergy among all of the FGC components are provided by the Scientific Director, who leads regular meetings with all Core Managers to discuss assay development, data analysis, trouble-shooting, integration, and new techniques. The Core Managers provide advice to investigators on experimental design prior to laboratory and clinical studies, assay development and quality control during the studies and interpretation of data generated by the Core. This "one-stop shopping" approach makes functional genomics research more broadly accessible to Cancer Center investigators. Thus, the FGC provides advanced technologies and support for basic, clinical and population studies that enhance functional genomics research and collaborations across all COHCCC programs. During the prior 12-month reporting period, the FGC was used by 14 Cancer Center members from all 5 Research Programs; peer-reviewed usage represented 76% of the total. The proposed total annual budget is $339,788, representing 39% institutional support, 18% user fees, 16% other, and 27% ($91,000) requested from the CCSG.