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Mining the Aspergillus nidulans Secondary Metabolome

挖掘构巢曲霉次级代谢组

基本信息

批准号：
8314135
负责人：
BERL Ray OAKLEY
金额：
$ 92.54万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-05-01 至 2015-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8314135
关键词：
Anabolism Aspergillus Aspergillus nidulans Biological Factors Biosynthesis of Secondary Metabolites Pathway California Chromatin Development Devices Family Funding Mechanisms Gene Cluster Gene Targeting Generations Genes Genome Genomics Geographic Locations Goals Head High Pressure Liquid Chromatography Interdisciplinary Study Laboratories Libraries Los Angeles Lovastatin Metabolism Methods Mining Molds Molecular Genetics Natural Products Chemistry Oceans Ohio Organism Pathway interactions Peptides Pharmaceutical Preparations Procedures Process Production Program Research Project Grants Public Health Publishing Regulation Research Research Personnel Screening procedure Secure Source Testing Universities Wisconsin Work analytical tool antimicrobial base clay frontier fungus gene replacement genome sequencing liquid chromatography mass spectrometry mutant novel novel strategies peptide synthase polyketide synthase programs promoter tool

项目摘要

The primary goal of this Program Project is to use a synergistic combination of genomics, molecular genetics and natural products chemistry to identify and purify potentially useful secondary metabolites of the filamentous fungus Aspergillus nidulans. The recently published sequencing of genomes of three species of Aspergillus has revealed that these species have a surprisingly large number of secondary metabolism pathways that are potential sources of new and useful natural products. The products of most of the A. nidulans secondary metabolism pathways are currently unknown and the three research groups will work together towards the goal of identifying and purifying the product(s) of each of the secondary metabolism gene clusters in A. nidulans. Project #1 is headed by Dr. Berl Oakley at Ohio State University, the overall P.I., who will develop tools for systematic promoter exchanges and for the systematic disruption of genes through targeted gene replacements. A complete library of A. nidulans strains carrying deletions of key nonribosomal peptide synthetase or polyketide synthase genes will be created. Project #2 is headed by Dr. Nancy Keller at the University of Wisconsin, who will focus on chromatin mutants to enhance the production of minimally expressed clusters and to activate "silent" clusters. Project #3 is headed by Dr. Clay Wang at the University of Southern California, who will focus on isolation and characterization of secondary metabolites produced by A. nidulans strains from the Oakley and Keller laboratories. In summary, this multidisciplinary collaboration will discover new secondary metabolites from Aspergillus nidulans and develop methods that can be used to mine the secondary metabolomes of other species of fungi for valuable natural products.

本项目的主要目标是利用基因组学、分子遗传学和天然产物化学的协同组合来鉴定和纯化丝状真菌细粒曲霉的潜在有用的次生代谢物。最近公布的三种曲霉的基因组测序显示，这些物种具有惊人的大量次级代谢途径，是新的和有用的天然产物的潜在来源。大部分A的产物。

项目成果

期刊论文数量（55）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Application of an efficient gene targeting system linking secondary metabolites to their biosynthetic genes in Aspergillus terreus.

DOI：
10.1021/ol401384v
发表时间：
2013-07-19
期刊：
ORGANIC LETTERS
影响因子：
5.2
作者：
Guo, Chun-Jun;Knox, Benjamin P.;Sanchez, James F.;Chiang, Yi-Ming;Bruno, Kenneth S.;Wang, Clay C. C.
通讯作者：
Wang, Clay C. C.

Expanding the Chemical Space of Nonribosomal Peptide Synthetase-like Enzymes by Domain and Tailoring Enzyme Recombination.

通过结构域扩展非核糖体肽合成酶样酶的化学空间和定制酶重组。