RADIATION DAMAGE AT DIFFERENT SPECIMEN TEMPERATURES

不同样本温度下的辐射损伤

• 批准号: 8361048
• 负责人: Wah Chiu
• 金额: $ 2.45万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361048
• 关键词: Attenuated; Biological; Cryoelectron Microscopy; Electron Microscopy; Freezing; Funding; Grant; Helium; Liquid substance; National Center for Research Resources; Nitrogen; Principal Investigator; Radiation; Research; Research Infrastructure; Resolution; Resources; Sampling; Source; Specimen; Temperature; United States National Institutes of Health; cost; cryogenics; macromolecule; radiation effect

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Radiation damage is the primary factor that limits resolution in electron cryo-microscopy (cryo-EM) of frozen-hydrated biological samples. Negative effects of radiation damage are attenuated by cooling specimens to cryogenic temperatures using liquid nitrogen or liquid helium.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 辐射损伤是限制低温电子显微镜分辨率的主要因素 冷冻-水合生物样品的冷冻-EM。辐射损伤的负面影响是 通过使用液氮或液体冷却样品至低温而衰减的 氦气