GLYCOPROTEINS EXPRESSING POLYSIALIC ACID AS MARKERS OF LOSS OF PLURIPOTENCY

表达多唾液酸的糖蛋白作为多能性丧失的标志物

• 批准号: 8363011
• 负责人: J. Michael Pierce
• 金额: $ 17.15万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363011
• 关键词: Antibodies; Binding; CD15 Antigens; Funding; Glycoproteins; Grant; Human; Mus; National Center for Research Resources; Pathway interactions; Polysaccharides; Polysialic Acid; Principal Investigator; Regulation; Research; Research Infrastructure; Resources; Source; Staging; Stem cells; Sum; Surface; Technology; Testing; Transcript; Transcriptional Regulation; United States National Institutes of Health; Up-Regulation; cost; dalton; glycosyltransferase; human embryonic stem cell; pluripotency; programs; protein expression; research study; technology development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Stem Cell Program (Dalton) has demonstrated that both mouse ESC and human ESC, when differentiated into all pathways tested thus far, begin to express polysialic acid (PSA) on their surfaces, as evidenced by anti-PSA antibody binding. This finding was suggested by changes in PSA synthetic glycosyltransferase transcript upregulation. The Subproject focuses on determining the mechanisms of expression of PSA during differentiation and its function. The scope of the subproject encompasses glycogene transcription regulation, acceptor protein expression, and development of technologies to identify the mechanism of regulation. In sum, this project demonstrates the utility of the Glycotranscriptomics Core in directing subsequent experiments to identify glycan changes during ESC differentiation. Intriguingly, both mouse and human differentiation pathways show similar glycan changes. This is by contrast to SSEA-1 and SSEA-3, two differentiation stage-specific glycans found during mouse ESC differentiation which are not found in human ESC differentiation.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 干细胞程序（道尔顿）已经证明，小鼠ESC和人ESC在迄今为止测试的所有途径中都开始在其表面上表达多氨酸（PSA），这是抗PSA抗体结合所证明的。 PSA合成糖基转移酶转录本上调的变化提出了这一发现。 该子项目的重点是确定分化过程中PSA表达的机理及其功能。 该子项目的范围包括糖基因转录调控，受体蛋白表达和开发技术以识别调节机理。 总而言之，该项目证明了糖术核心核心在指导后续实验以识别ESC分化过程中的聚糖变化时的实用性。 有趣的是，小鼠和人类分化途径都显示出相似的聚糖变化。 这与SSEA-1和SSEA-3相反，在小鼠ESC分化过程中发现了两个分化阶段特异性的聚糖，这在人类ESC分化中未发现。