IDENTIFICATION OF POTENTIAL GLYCAN MARKERS OF MOUSE MAMMARY CANCER STEM CELLS

小鼠乳腺癌干细胞潜在聚糖标记物的鉴定

• 批准号: 8363122
• 负责人: J. Michael Pierce
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363122
• 关键词: Cells; Development; Flow Cytometry; Funding; Grant; Mammary Neoplasms; Mouse Mammary Tumor Virus; Mus; National Center for Research Resources; Oncogenes; Polyomavirus; Polysaccharides; Principal Investigator; Research; Research Infrastructure; Resources; Source; Technology; United States National Institutes of Health; cancer stem cell; cost; stem cell population; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Using MMTV-polyoma middle-T oncogene expressing mice, we have used Aldefluor positivity to isolate the cancer stem cell population from mature tumors. We will characterize the glycome of these cells in comparison to the non-cancer stem cell population (Aldefluor negative by flow cytometry) by applying the technologies we have developed, glycotranscriptomics and glycan analysis, and those in development, including SRM.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 使用表达MMTV-多瘤中T癌基因的小鼠，我们利用Aldeflor阳性从成熟肿瘤中分离出癌症干细胞群。 我们将通过应用我们已开发的技术、糖转录组学和聚糖分析以及包括 SRM 在内的正在开发的技术，与非癌症干细胞群（流式细胞术检测为 Aldeflu 阴性）相比，对这些细胞的糖组进行表征。