IDENTIFICATION OF POTENTIAL GLYCAN MARKERS OF MOUSE MAMMARY CANCER STEM CELLS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 利用表达MMTV-多瘤中T癌基因的小鼠，我们利用Aldeflor阳性从成熟肿瘤中分离出肿瘤干细胞群。我们将通过应用我们开发的技术、糖转录学和糖链分析以及包括SRM在内的正在开发的技术，将这些细胞的糖类与非癌症干细胞群体(流式细胞仪Aldefluor阴性)进行比较。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Using MMTV-polyoma middle-T oncogene expressing mice, we have used Aldefluor positivity to isolate the cancer stem cell population from mature tumors. We will characterize the glycome of these cells in comparison to the non-cancer stem cell population (Aldefluor negative by flow cytometry) by applying the technologies we have developed, glycotranscriptomics and glycan analysis, and those in development, including SRM.