IDENTIFICATION OF POTENTIAL GLYCAN MARKERS OF MOUSE MAMMARY CANCER STEM CELLS

小鼠乳腺癌干细胞潜在聚糖标记物的鉴定

• 批准号: 8363122
• 负责人: J. Michael Pierce
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363122
• 关键词: Cells; Development; Flow Cytometry; Funding; Grant; Mammary Neoplasms; Mouse Mammary Tumor Virus; Mus; National Center for Research Resources; Oncogenes; Polyomavirus; Polysaccharides; Principal Investigator; Research; Research Infrastructure; Resources; Source; Technology; United States National Institutes of Health; cancer stem cell; cost; stem cell population; tumor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Using MMTV-polyoma middle-T oncogene expressing mice, we have used Aldefluor positivity to isolate the cancer stem cell population from mature tumors. We will characterize the glycome of these cells in comparison to the non-cancer stem cell population (Aldefluor negative by flow cytometry) by applying the technologies we have developed, glycotranscriptomics and glycan analysis, and those in development, including SRM.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 使用MMTV-多瘤中T癌基因表达小鼠，我们使用Aldefluor阳性从成熟肿瘤中分离癌症干细胞群。 我们将通过应用我们开发的技术、糖转录组学和聚糖分析以及正在开发的技术（包括SRM），与非癌症干细胞群体（流式细胞术检测为Aldefluor阴性）相比，表征这些细胞的糖组。