MOUSE BRAIN GLYCOPROTEINS EXPRESSING O-MAN AND ASN-LINKED GLYCANS

表达 O-MAN 和 ASN 连接聚糖的小鼠脑糖蛋白

• 批准号: 8363012
• 负责人: J. Michael Pierce
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363012
• 关键词: Brain; Cell-Cell Adhesion; Enzymes; Funding; Glycoproteins; Grant; Knockout Mice; Link; Mannose; Mass Spectrum Analysis; Mus; National Center for Research Resources; Neurons; Polysaccharides; Principal Investigator; Research; Research Infrastructure; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Techniques; Technology; United States National Institutes of Health; brain tissue; cell motility; cost; glycosyltransferase; man; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A relatively new type of O-linked glycan has been implicated in neuronal cell adhesion and migration, the O-linked mannose glycans. This project will use novel techniques to separate glycoproteins expressing O-Man glycans from brain tissue and identify these glycoproteins using mass spectrometry techniques. In addition, the Asn-linked glycans from mouse brain have been identified by MALDI-TOF MS. These are being compared to glycans found in mice that are null for the GnT-Va glycosyltransferase to determine how this enzyme regulates glycoprotein glycan expression. A GnT-Vb null mouse has now been produced and the effects of this deletion on O-Man and Asn-linked glycoproteins is now under study.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 一种相对较新的 O-连接聚糖，即 O-连接甘露糖聚糖，与神经元细胞粘附和迁移有关。 该项目将使用新技术从脑组织中分离表达 O-Man 聚糖的糖蛋白，并使用质谱技术鉴定这些糖蛋白。此外，MALDI-TOF MS 还鉴定了小鼠大脑中的 Asn 连接聚糖。将它们与在 GnT-Va 糖基转移酶无效的小鼠中发现的聚糖进行比较，以确定该酶如何调节糖蛋白聚糖的表达。 现已培育出 GnT-Vb 缺失小鼠，并且目前正在研究这种缺失对 O-Man 和 Asn 连接糖蛋白的影响。