MOUSE BRAIN GLYCOPROTEINS EXPRESSING O-MAN AND ASN-LINKED GLYCANS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 一种相对较新的O-连接多糖与神经细胞的黏附和迁移有关，即O-连接甘露聚糖。该项目将使用新技术从脑组织中分离表达O-Man多糖的糖蛋白，并使用质谱学技术鉴定这些糖蛋白。此外，还通过MALDI-TOF MS鉴定了小鼠脑中与ASN连接的多聚糖，并将其与在小鼠中发现的GnT-Va糖基转移酶阴性的多聚糖进行比较，以确定该酶如何调节糖蛋白多糖的表达。现在已经产生了一只GnT-VB缺失的小鼠，这种缺失对O-Man和ASN连接的糖蛋白的影响现在正在研究中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A relatively new type of O-linked glycan has been implicated in neuronal cell adhesion and migration, the O-linked mannose glycans. This project will use novel techniques to separate glycoproteins expressing O-Man glycans from brain tissue and identify these glycoproteins using mass spectrometry techniques. In addition, the Asn-linked glycans from mouse brain have been identified by MALDI-TOF MS. These are being compared to glycans found in mice that are null for the GnT-Va glycosyltransferase to determine how this enzyme regulates glycoprotein glycan expression. A GnT-Vb null mouse has now been produced and the effects of this deletion on O-Man and Asn-linked glycoproteins is now under study.