Multi-modal AO-LSO Phase Gradient Imaging of the Inner Retina

内视网膜多模态 AO-LSO 相位梯度成像

• 批准号: 8524620
• 负责人: R DANIEL FERGUSON
• 金额: $ 20.32万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8524620
• 关键词: Address; Algorithms; Alzheimer' s Disease; Apoptosis; Area; Back; Biological Process; Blood Vessels; Boston; Cellular Structures; Clinic; Clinical; Clinical Investigator; Clinical Research; Collaborations; Coupling; Detection; Development; Diabetic Retinopathy; Diagnosis; Discrimination; Disease; Disease Progression; Engineering; Evaluation; Eye; Eye diseases; Feedback; Floods; Flying body movement; Fundus; Glaucoma; Goals; Government; Human; Human Volunteers; Image; Lasers; Lateral; Lead; Left; Life; Light; Lighting; Masks; Measures; Methods; Microscopy; Monitor; Nerve Degeneration; Neurons; Ophthalmoscopes; Ophthalmoscopy; Optics; Pathology; Patients; Pattern; Pediatric Hospitals; Persons; Pharmacotherapy; Phase; Phase-Contrast Microscopy; Photophobia; Photoreceptors; Physiological; Positioning Attribute; Process; Proxy; Pupil; Reading; Relative (related person); Research; Research Personnel; Resolution; Retina; Retinal; Retinal Cone; Retinal Degeneration; Retinal Ganglion Cells; Retinopathy of Prematurity; Scanning; Scheme; Scientist; Side; Source; Speed; Spottings; Staging; System; Technology; Testing; Thick; Time; Tissues; Vision; Vision research; adaptive optics; base; cellular imaging; clinical application; design; drug discovery; flexibility; imaging modality; improved; in vivo; in vivo imaging; insight; instrument; instrumentation; neuronal cell body; novel; novel strategies; optical imaging; physical science; programs; prototype; public health relevance; receptor; relating to nervous system; retinal nerve fiber layer; retinal neuron; sensor; tool; transmission process; volunteer

Project Summary/Abstract Powerful new Adapative Optics imaging technoIogies which sense ocular aberrations with a wave- front sensor and corrects them with a deformable mirror wave-front compensator, like Adaptive Optics Scanning Laser Ophthalmoscopy (AOSLO) and AO-OCT, provide detailed anatomical, physiological and functional information at the cellular level in the human eye. Physical Sciences Inc. (PSI) has recently demonstrated a new approach, called adaptive optics line scanning ophthalmoscopy (AO-LSO). The clinical AO-LSO retinal imager, invented and developed at PSI, greatly simplifies AO optical design, eliminates high-speed scanning components, and reduces the clinical footprint compared to research AOSLOs, while preserving many of the advantages of (line-)confocal imaging for resolving retinal layers. Although the AOSLO and AO-LSO provide the ability to routinely image and count cone photoreceptors, at present, there are no direct approaches for in vivo imaging of retinal ganglion cells in the human eye, or counting cell bodies in the inner retina. Our goal in Phase I is to demonstrate an advanced new design of the AO-LSO imager, (including an AO-OCT channel), with a new, high-sensitivity camera technology and line-illumination methods for phase gradient imaging of the inner retina. The envisioned platform will be engineered as a compact clinical system for flexible cellular imaging applications in vivo. An AO-LSO prototype instrument will be modified with new line-camera technology in Phase I, and used to investigate new imaging modalities. If successful, this approach and associated imaging algorithms will be improved in Phase II and used to monitor disease progression/regression and characterize inner retinal remodeling in patients at Children's Hospital Boston. A clinical investigator will provide feedback in Phase I and will lead a Phase II clinical study to evaluate the new capabilities of the phase gradient imaging approach. Phase III commercial development will provide clinicians with enhanced adaptive optics imaging capabilities not available in current retinal imagers.

项目摘要/摘要 强大的自适应光学成像技术，可以通过波检测眼睛的像差- 并使用可变形镜波前补偿器进行校正，如自适应光学 扫描激光眼底镜(AOSLO)和AO-OCT，提供详细的解剖、生理和 人眼细胞水平的功能信息。物理科学公司(PSI)最近 展示了一种新的方法，称为自适应光学行扫描眼底镜(AO-LSO)。这个 PSI发明和开发的临床型AO-LSO视网膜成像仪极大地简化了光学设计， 与研究相比，消除了高速扫描组件，并减少了临床占用空间 AOSLOS，同时保留了(线)共焦成像在分辨视网膜层方面的许多优势。 虽然AOSLO和AO-LSO提供了常规成像和计数视锥感光细胞的能力，但在 目前，还没有直接的方法对人眼的视网膜神经节细胞进行活体成像，或者 计数视网膜内侧的细胞体。我们在第一阶段的目标是展示先进的新设计 AO-LSO成像仪(包括一个AO-OCT通道)，采用新的高灵敏度相机技术和 视网膜内层位相梯度成像的线照明方法。设想的平台将是 作为一种紧凑型临床系统设计，用于活体内灵活的细胞成像应用。一个AO-LSO 在一期工程中，将对样机进行改装，采用新的线阵摄像技术，用于研究 新的成像模式。如果成功，这种方法和相关的成像算法将在 II期，用于监测疾病进展/消退和表征视网膜内重塑 波士顿儿童医院的病人。临床调查员将在第一阶段提供反馈，并将领导一项 第二阶段临床研究，以评估相位梯度成像方法的新能力。第三阶段 商业开发将为临床医生提供增强的自适应光学成像能力，而不是 在目前的视网膜成像仪中可用。