Adaptive Optics OCT/Fluorescence Small Animal Imager

自适应光学 OCT/荧光小动物成像仪

• 批准号: 8715018
• 负责人: R DANIEL FERGUSON
• 金额: $ 22.07万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8715018
• 关键词: Affect; Anatomy; Angiography; Animal Experimentation; Animal Model; Animals; Anterior; Automation; Basic Science; Beds; Biological Markers; Blood Vessels; Boston; Characteristics; Choroid; Computer software; Contrast Media; Control Animal; Development; Devices; Diabetes Mellitus; Diabetic Retinopathy; Diagnostic; Diagnostic Imaging; Disease; Elements; Eye; Eye diseases; Fluorescence; Functional Imaging; Funding; Fundus; Glaucoma; Goals; Health; Human; Image; Imaging Device; Inflammatory; Inherited; Institution; Investigation; Laboratories; Lasers; Lead; Length; Life; Marketing; Measures; Methods; Microglia; Microscope; Modeling; Molecular Conformation; Monitor; Multimodal Imaging; Mus; Pathogenesis; Pathology; Pediatric Hospitals; Phase; Process; Research; Resolution; Retina; Retinal; Retinal Degeneration; Rodent; Rodent Model; Scanning; Site; Source; Speed; Streptozocin; Structure; System; Technology; Testing; Thick; Time; Transgenic Animals; Transgenic Mice; Vendor; Vision; Vision Disorders; Vision research; adaptive optics; arm; base; commercialization; design; drug discovery; experience; flexibility; fluorescence imaging; image registration; imaging modality; improved; in vivo; in vivo imaging; innovation; mouse model; novel; novel diagnostics; photonics; physical science; programs; prototype; relating to nervous system; research study; tool

DESCRIPTION (provided by applicant): Physical Sciences Inc. proposes to develop a new, integrated multimodal adaptive optics small animal imager, including AO-OCT and AO scanning laser fluorescence (fAOSLO), and to demonstrate its applications to detecting pathology in the retinas of rodent models of eye disease. The imaging platform includes novel design elements such as adaptable wavefront sensing methods enabling dynamic and static AO correction in selected layers; integrated Badal optometer system with path-length-locked OCT reference arm for flexible AO-OCT-guided imaging, and co-scanning AO-OCT/fAOSLO rasters for enhanced fluorescence sensitivity and co-registration in anesthetized rodents. Together with collaborators at Children's Hospital Boston (CHB), we propose to demonstrate the imaging platform in a GFP mouse (B6.129P-Cx3cr1tm1Litt/J mice) model of STZ-induced diabetic retinopathy. The rapidly growing array of small animal models of human eye disease has significantly increased the need for new in vivo animal fundus imaging modalities with cellular resolution. However, there are currently no commercial AO platforms for high resolution in vivo imaging which can exploit such features. A successful Phase I program will result in a multimodal adaptive optics small animal imaging (MAOSI) platform with simultaneous AO- OCT, fAOSLO, and improved AO control for 3D structural and functional imaging. Such a device has immediate research and drug discovery applications and markets.

描述（由申请人提供）：Physical Sciences Inc.提议开发一种新型集成多模态自适应光学小动物成像仪，包括AO-OCT和AO扫描激光荧光（fAOSLO），并展示其在检测啮齿动物眼病模型视网膜病理学方面的应用。该成像平台包括新颖的设计元素，例如自适应波前传感方法，可在选定层中进行动态和静态 AO 校正；集成 Badal 验光计系统，具有路径长度锁定 OCT 参考臂，可实现灵活的 AO-OCT 引导成像，并可共同扫描 AO-OCT/fAOSLO 光栅，以增强麻醉啮齿动物的荧光灵敏度和共同配准。我们与波士顿儿童医院 (CHB) 的合作者一起，建议在 STZ 诱导的糖尿病视网膜病变的 GFP 小鼠（B6.129P-Cx3cr1tm1Litt/J 小鼠）模型中展示成像平台。人眼疾病小动物模型的快速增长显着增加了对具有细胞分辨率的新型体内动物眼底成像模式的需求。然而，目前还没有用于高分辨率体内成像的商业 AO 平台可以利用这些功能。成功的第一阶段计划将产生一个多模态自适应光学小动物成像 (MAOSI) 平台，该平台具有同步 AO-OCT、fAOSLO 以及改进的 3D 结构和功能成像 AO 控制。这种设备具有直接的研究和药物发现应用和市场。