AO-based High Resolution Retinal Imager for Rodent Eye

项目摘要/摘要 物理科学公司(PSI)建议开发和演示我们的多模式车的新原型 适合商业化的自适应光学小动物成像(MAOSI)平台。MAOSI是一家 灵活的高分辨率(1微米)台式视网膜成像系统，具有多达四个共焦反射比 自适应光学扫描光检眼镜(RAOSLO)和/或荧光AOSLO(FAOSLO) 具有同步AO-OCT的通道，用于动物研究中的常规实验室成像应用。小说 平台设计元素包括以下特点：独立软件控制的焦深 RAOSLO和fAOSLO通道，具有近衍射极限轴向分辨率(&lt；10微米)；高动态范围 具有动态声光校正的时间门控波前传感；三振镜扫描引擎，具有 软件可调扫描图案，具有2度至30度可移动的栅格；可选择扫描物镜 和瞳孔大小(1到3 mm)的新型小动物舞台上。同步、共扫描的AO-OCT/SLO 栅格在图像制导、跨模式配准和平均 增强了在麻醉动物中的检测灵敏度和易用性。第二阶段平台将 融合了明场、暗场和多孔径AOSLO模式， 多芯光纤电缆分布到多个探测器，以改进相位梯度成像。该系统是 可针对多个离散激光和SLD源或单个商业超连续谱进行配置 源(SCS)以实现最大灵活性。可调谐的SDOCT光谱仪和配备SCS的平台将 在选定的可见波长范围内实现各种新颖的光谱测量，并提供新的 要纳入的功能模式。使用弱光的能力，以及使用接触 镜片和动物体温保持在定制的动物舞台/支架上，防止损坏 减少角膜和晶状体混浊。

Project Summary/Abstract Physical Sciences Inc. (PSI) proposes to develop and demonstrate a new prototype of our Multimodal Adaptive Optics Small Animal Imaging (MAOSI) platform suitable for commercialization. MAOSI is a flexible, high-resolution (<1µm) benchtop retinal imaging system with up to four confocal reflectance Adaptive Optics Scanning Light Ophthalmoscope (rAOSLO) and/or fluorescence AOSLO (fAOSLO) channels with simultaneous AO-OCT for routine laboratory imaging applications in animal research. Novel platform design elements includes such features as: independent software-controlled focal depths for rAOSLO and fAOSLO channels with near-diffraction limited axial resolution (<10µm); high dynamic range time-gated wavefront sensing with dynamic AO correction; a three-galvanometer scan engine with software-adjustable scan patterns with moveable rasters from 2 deg to 30 deg; selectable scan objectives and pupil sizes (1 to 3mm) on a novel small animal stage. Simultaneous, co-scanning AO-OCT/SLO rasters provide significant benefits for image guidance, cross-modal registration and averaging for enhanced detection sensitivity and ease of use in anesthetized animals. The Phase II platform will incorporate bright field, dark field and multi-aperture AOSLO modalities easily configured by multi-aperture, multi-core fiber cable distribution to multiple detectors for improved phase gradient imaging. The system is configurable either for multiple discrete laser and SLD sources or for a single commercial supercontinuum source (SCS) for maximum flexibility. A tunable SDOCT spectrometer and SCS-equipped platform will enable a wide variety of novel spectral measurements in selected visible wavelength bands and new functional modalities to be incorporated. The ability to use low light levels, along with the use of contact lenses and animal body temperature maintenance in a custom animal stage/holder, prevent damage to retina and minimize cornea and lens opacifications.