AO-based High Resolution Retinal Imager for Rodent Eye

基于 AO 的啮齿动物眼高分辨率视网膜成像仪

• 批准号: 9347969
• 负责人: R DANIEL FERGUSON
• 金额: $ 49.41万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9347969
• 关键词: Anatomy; Animal Disease Models; Animal Experimentation; Animal Model; Animals; Automation; Behavior; Biological Markers; Blood Vessels; Blood flow; Body Temperature; Boston; Cells; Characteristics; Choroid; Computer software; Contact Lenses; Cornea; Custom; Darkness; Detection; Development; Diagnostic Imaging; Disease; Disease Progression; Disease model; Elements; Engineering; Evaluation; Eye; Eye diseases; Fiber; Fluorescence; Functional Imaging; Goals; Government; Image; Imaging Device; Inflammatory; Institution; Investigation; Label; Laboratories; Lasers; Light; Maintenance; Measurement; Metabolic; Methods; Modality; Modeling; Molecular; Monitor; Morphology; Mus; Ophthalmoscopes; Optics; Pathologic; Pathology; Pattern; Pediatric Hospitals; Persons; Phase; Process; Property; Protocols documentation; Pupil; Rattus; Research; Resolution; Retina; Retinal; Rodent; Sales; Scanning; Scheme; Small Animal Imaging Systems; Source; Speed; Structure; System; Technology; Testing; Thick; Time; Transgenic Animals; Vision; adaptive optics; adaptive optics scanning laser ophthalmoscopy; animal imaging; base; commercialization; design; detector; drug discovery; experimental study; flexibility; fluorophore; human imaging; image guided; imaging capabilities; imaging modality; imaging platform; imaging program; imaging system; improved; in vivo imaging; instrument; lens; mouse model; multimodality; novel; novel therapeutics; physical science; prevent; programs; prototype; relating to nervous system; retinal damage; software systems; success

Project Summary/Abstract Physical Sciences Inc. (PSI) proposes to develop and demonstrate a new prototype of our Multimodal Adaptive Optics Small Animal Imaging (MAOSI) platform suitable for commercialization. MAOSI is a flexible, high-resolution (<1µm) benchtop retinal imaging system with up to four confocal reflectance Adaptive Optics Scanning Light Ophthalmoscope (rAOSLO) and/or fluorescence AOSLO (fAOSLO) channels with simultaneous AO-OCT for routine laboratory imaging applications in animal research. Novel platform design elements includes such features as: independent software-controlled focal depths for rAOSLO and fAOSLO channels with near-diffraction limited axial resolution (<10µm); high dynamic range time-gated wavefront sensing with dynamic AO correction; a three-galvanometer scan engine with software-adjustable scan patterns with moveable rasters from 2 deg to 30 deg; selectable scan objectives and pupil sizes (1 to 3mm) on a novel small animal stage. Simultaneous, co-scanning AO-OCT/SLO rasters provide significant benefits for image guidance, cross-modal registration and averaging for enhanced detection sensitivity and ease of use in anesthetized animals. The Phase II platform will incorporate bright field, dark field and multi-aperture AOSLO modalities easily configured by multi-aperture, multi-core fiber cable distribution to multiple detectors for improved phase gradient imaging. The system is configurable either for multiple discrete laser and SLD sources or for a single commercial supercontinuum source (SCS) for maximum flexibility. A tunable SDOCT spectrometer and SCS-equipped platform will enable a wide variety of novel spectral measurements in selected visible wavelength bands and new functional modalities to be incorporated. The ability to use low light levels, along with the use of contact lenses and animal body temperature maintenance in a custom animal stage/holder, prevent damage to retina and minimize cornea and lens opacifications.

项目总结/摘要 物理科学公司(PSI)建议开发和展示我们的多式联运的新原型 适合商业化的自适应光学小动物成像（MAOSI）平台。MAOSI是一个 灵活的高分辨率（<1µm）台式视网膜成像系统，具有多达四个共焦反射 自适应光学扫描光检眼镜（rAOSLO）和/或荧光AOSLO（fAOSLO） 通道与同步AO-OCT配合使用，用于动物研究中的常规实验室成像应用。小说 平台设计要素包括如下特征：独立的软件控制的焦深， rAOSLO和fAOSLO通道，具有近衍射极限的轴向分辨率（<10µm）;高动态范围 具有动态AO校正的时间选通波前传感;三振镜扫描引擎， 软件可调扫描模式，光栅可从2 °移动到30 °;可选扫描物镜 和瞳孔大小（1 ~ 3 mm）的动物模型。同步、共扫描AO-OCT/SLO 光栅为图像引导、交叉模态配准和平均化提供了显著的优势， 提高了检测灵敏度并易于在麻醉动物中使用。第二阶段平台将 结合明场、暗场和多孔径AOSLO模式， 多芯光纤电缆分布到多个检测器，用于改善相位梯度成像。该系统 可配置用于多个离散激光器和SLD源或用于单个商业超连续谱 源（SCS）以获得最大的灵活性。可调谐SDOCT光谱仪和配备SCS的平台将 能够在选定的可见光波段进行各种新颖的光谱测量， 将纳入的功能模式。能够使用低光水平，沿着使用接触 在定制的动物载物台/保持器中保持镜片和动物体温， 视网膜和减少角膜和透镜混浊。