Low-Coherence Wavefront Sensor for AO Imaging in Rodent Eyes

用于啮齿动物眼睛 AO 成像的低相干波前传感器

• 批准号: 7529346
• 负责人: R DANIEL FERGUSON
• 金额: $ 2.17万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-30 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7529346
• 关键词: Algorithms; Animal Experimentation; Animals; Architecture; Astigmatism; Behavior; Benchmarking; Closure; Coma; Computer software; Dependence; Depth; Devices; Diagnostic Imaging; Disadvantaged; Evaluation; Eye; Eye diseases; Failure; Frequencies; Fundus; Goals; Government; Histology; Human; Human Resources; Image; Imaging Techniques; Knowledge; Lead; Life; Light; Maps; Measurement; Measures; Methods; Microscope; Microscopy; Modality; Mus; Ophthalmoscopes; Optics; Performance; Persons; Pharmacologic Substance; Phase; Positioning Attribute; Process; Purpose; Range; Rate; Relative (related person); Research; Residual state; Resolution; Retina; Rodent; Scanning; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Source; Stroke; System; Technology; Testing; Thick; Time; Tissues; Transgenic Mice; Transgenic Organisms; Upper arm; Ursidae Family; abstracting; adaptive optics; base; design; desire; digital imaging; experience; image processing; improved; in vivo; interest; mouse model; optical imaging; physical science; programs; sensor; tool; vector; virtual

Project Summary/Abstract The increasing importance of transgenic mouse models of human eye disease makes in vivo fundus imaging modalities at cellular resolution increasingly desirable. Due to the poor optical quality of rodent eyes, there are few viable methods for high resolution imaging. This can lead to greater dependence on more costly and time-consuming histology where it otherwise might not be necessary if adequate imaging techniques were readily available. Adaptive Optics (AO) deformable mirror (DM) technologies of several types have matured to the point where the stroke and resolution may be adequate to compensate large rodent eye aberrations. However, the currently preferred Shack-Hartmann wavefront sensing technology may have reached a practical limit for extracting high order aberrations sufficient for diffraction-limited AO performance in rodent eyes. The goal of the Phase I program is to develop and test key aspects of an axially resolved, low-coherence wavefront sensing method based on a simple interferometric design to measure high order aberrations in rodent eyes. We propose to show that this device is complementary to the Shack-Hartmann device with a dual mode test system in well-characterized phantom eyes.

项目摘要/摘要 人类眼病转基因小鼠模型的日益重要性使得活体眼底 细胞分辨率的成像方式越来越受欢迎。由于啮齿动物的光学质量较差 眼睛，几乎没有可行的方法来进行高分辨率成像。这可能会导致对 更昂贵和更耗时的组织学检查，如果有足够的成像，否则可能不需要 技术是现成的。自适应光学(AO)变形镜(DM)技术 类型已经成熟到笔划和分辨率可能足以补偿大的 啮齿动物的眼睛像差。然而，目前首选的夏克-哈特曼波前传感技术 可能已经达到了提取高阶像差的实用极限，足以满足衍射受限的声光 在啮齿动物眼中的表现。第一阶段计划的目标是开发和测试 一种基于简单干涉设计的轴向分辨、低相干波前传感方法 测量啮齿动物眼睛的高阶像差。我们打算证明这个装置是对 Shack-Hartmann设备在具有良好特征的幻影眼睛中采用了双模式测试系统。