Low-Coherence Wavefront Sensor for AO Imaging in Rodent Eyes

用于啮齿动物眼睛 AO 成像的低相干波前传感器

• 批准号: 7529346
• 负责人: R DANIEL FERGUSON
• 金额: $ 2.17万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-30 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7529346
• 关键词: Algorithms; Animal Experimentation; Animals; Architecture; Astigmatism; Behavior; Benchmarking; Closure; Coma; Computer software; Dependence; Depth; Devices; Diagnostic Imaging; Disadvantaged; Evaluation; Eye; Eye diseases; Failure; Frequencies; Fundus; Goals; Government; Histology; Human; Human Resources; Image; Imaging Techniques; Knowledge; Lead; Life; Light; Maps; Measurement; Measures; Methods; Microscope; Microscopy; Modality; Mus; Ophthalmoscopes; Optics; Performance; Persons; Pharmacologic Substance; Phase; Positioning Attribute; Process; Purpose; Range; Rate; Relative (related person); Research; Residual state; Resolution; Retina; Rodent; Scanning; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Source; Stroke; System; Technology; Testing; Thick; Time; Tissues; Transgenic Mice; Transgenic Organisms; Upper arm; Ursidae Family; abstracting; adaptive optics; base; design; desire; digital imaging; experience; image processing; improved; in vivo; interest; mouse model; optical imaging; physical science; programs; sensor; tool; vector; virtual

Project Summary/Abstract The increasing importance of transgenic mouse models of human eye disease makes in vivo fundus imaging modalities at cellular resolution increasingly desirable. Due to the poor optical quality of rodent eyes, there are few viable methods for high resolution imaging. This can lead to greater dependence on more costly and time-consuming histology where it otherwise might not be necessary if adequate imaging techniques were readily available. Adaptive Optics (AO) deformable mirror (DM) technologies of several types have matured to the point where the stroke and resolution may be adequate to compensate large rodent eye aberrations. However, the currently preferred Shack-Hartmann wavefront sensing technology may have reached a practical limit for extracting high order aberrations sufficient for diffraction-limited AO performance in rodent eyes. The goal of the Phase I program is to develop and test key aspects of an axially resolved, low-coherence wavefront sensing method based on a simple interferometric design to measure high order aberrations in rodent eyes. We propose to show that this device is complementary to the Shack-Hartmann device with a dual mode test system in well-characterized phantom eyes.

项目总结/摘要 转基因小鼠模型在人类眼病研究中的重要性日益增加， 越来越需要细胞分辨率的成像模式。由于啮齿动物的光学质量差， 对于眼睛来说，几乎没有可行的高分辨率成像方法。这可能会导致更大的依赖 更昂贵和耗时的组织学检查，如果有足够的成像， 技术是现成的。自适应光学（AO）变形镜（DM）技术的几个 类型已经成熟到这样的程度，即冲程和分辨率可能足以补偿大的 啮齿类动物的眼畸变然而，目前优选的Shack-Hartmann波前传感技术 可能已经达到了提取足以用于衍射限制AO的高阶像差的实际极限 在啮齿动物眼中的表现第一阶段计划的目标是开发和测试 一种基于简单干涉设计的轴向分辨的低相干波前传感方法， 测量啮齿动物眼睛的高阶像差。我们建议表明，该设备是互补的， Shack-Hartmann装置与双模式测试系统在良好表征的体模眼中。