Low-Coherence Wavefront Sensor for AO Imaging in Rodent Eyes

用于啮齿动物眼睛 AO 成像的低相干波前传感器

• 批准号: 7326947
• 负责人: R DANIEL FERGUSON
• 金额: $ 14.32万
• 依托单位: PHYSICAL SCIENCES, INC
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-30 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7326947
• 关键词: Algorithms; Animal Experimentation; Animals; Architecture; Astigmatism; Behavior; Benchmarking; Closure; Coma; Computer software; Dependence; Depth; Devices; Diagnostic Imaging; Disadvantaged; Evaluation; Eye; Eye diseases; Failure; Frequencies; Fundus; Goals; Government; Histology; Human; Human Resources; Image; Imaging Techniques; Knowledge; Lead; Life; Light; Maps; Measurement; Measures; Methods; Microscope; Microscopy; Modality; Mus; Ophthalmoscopes; Optics; Performance; Persons; Pharmacologic Substance; Phase; Positioning Attribute; Process; Purpose; Range; Rate; Relative (related person); Research; Residual state; Resolution; Retina; Rodent; Scanning; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Source; Stroke; System; Technology; Testing; Thick; Time; Tissues; Transgenic Mice; Transgenic Organisms; Upper arm; Ursidae Family; adaptive optics; base; design; desire; digital imaging; experience; image processing; improved; in vivo; interest; mouse model; optical imaging; physical science; programs; sensor; tool; vector; virtual

DESCRIPTION (provided by applicant): The increasing importance of transgenic mouse models of human eye disease makes in vivo fundus imaging modalities at cellular resolution increasingly desirable. Due to the poor optical quality of rodent eyes, there are few viable methods for high resolution imaging. This can lead to greater dependence on more costly and time-consuming histology where it otherwise might not be necessary if adequate imaging techniques were readily available. Adaptive Optics (AO) deformable mirror (DM) technologies of several types have matured to the point where the stroke and resolution may be adequate to compensate large rodent eye aberrations. However, the currently preferred Shack-Hartmann wavefront sensing technology may have reached a practical limit for extracting high order aberrations sufficient for diffraction-limited AO performance in rodent eyes. The goal of the Phase I program is to develop and test key aspects of an axially resolved, low-coherence wavefront sensing method based on a simple interferometric design to measure high order aberrations in rodent eyes. We propose to show that this device is complementary to the Shack-Hartmann device with a dual mode test system in well-characterized phantom eyes.

描述（由申请人提供）：人类眼病的转基因小鼠模型的重要性日益增加，使得细胞分辨率的体内眼底成像模式日益可取。由于啮齿动物眼睛的光学质量差，几乎没有可行的方法用于高分辨率成像。这可能导致更大程度上依赖于更昂贵和耗时的组织学，否则，如果有足够的成像技术，就不需要组织学。几种类型的自适应光学（AO）可变形镜（DM）技术已经成熟到行程和分辨率可能足以补偿大的啮齿动物眼睛像差的程度。然而，当前优选的Shack-Hartmann波前感测技术可能已经达到了用于提取足以用于啮齿动物眼睛中的衍射受限AO性能的高阶像差的实际极限。第一阶段计划的目标是开发和测试基于简单干涉设计的轴向分辨、低相干波前传感方法的关键方面，以测量啮齿动物眼睛中的高阶像差。我们建议表明，该设备是互补的Shack-Hartmann设备的双模测试系统，在良好的特点幻影眼睛。