Arsenic Uptake, Transport and Storage in Plants

砷在植物中的吸收、运输和储存

• 批准号: 8650448
• 负责人: MARY LOU GUERINOT
• 金额: $ 37.5万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8650448
• 关键词: Ablation; Affect; Angiosperms; Arabidopsis; Arsenates; Arsenic; Arsenites; Blood Vessels; Breeding; Candidate Disease Gene; Carcinogens; Cereals; Characteristics; Chemicals; Chromosome Mapping; Collection; Complement; Coupled; Cross-Sectional Studies; Data; Detection; Development; Diet; Edible Plants; Electron Probe Microanalysis; Elements; Eligibility Determination; Exposure to; Ferns; Floods; Fluorescence; Food; Funding; Genes; Genetic; Genetic Screening; Genetic Variation; Goals; Homeostasis; Human; Image; Lasers; Location; Maps; Mass Spectrum Analysis; Mercury; Metals; Microarray Analysis; Movement; Organ; Oryza sativa; Oxidoreductase; Pathway interactions; Pesticides; Phenotype; Plant Roots; Plant Sources; Plants; Plasma; Population; Problem Solving; Process; Production; Pteris; Quantitative Trait Loci; RNA Interference; Relative (related person); Resolution; Rice; Roentgen Rays; Seeds; Side; Silicon; Soil; Source; Spectrum Analysis; Synchrotrons; System; Techniques; Testing; Tissues; Transgenic Organisms; Variant; Vascular System; Work; absorption; abstracting; base; beamline; cost effective; early life exposure; genetic analysis; genome wide association study; improved; in vivo; innovation; loss of function; molecular marker; next generation sequencing; oxidation; plant growth/development; prevent; programs; research study; stem; submicron; tool; transcriptome sequencing; uptake

Project 1: Abstract Rice (Oryza sativa), a staple food for over half the world's population, represents a significant dietary source of inorganic arsenic (As), a non-threshold, class 1 human carcinogen. It is imperative that strategies to reduce grain As are developed, and establishing the mechanisms that enable As to reach and accumulate within the rice grain is key to this endeavor. This project will elucidate the genetic control of As homeostasis in plants to enable the development of plants that do not accumulate As. The first specific aim tests the hypothesis that genetic diversity in rice can be exploited to lower grain As levels by mapping genes in accessions known to vary in grain As content. The second aim asks more generally what genes are involved in transferring As to the grain and the third aim tests strategies to limit the movement of As to the shoot/grain based on information gained from study of rice, Arabidopsis and the As accumulating fern Pteris vittata. The long-term goal is to prevent As accumulation in the edible portion of rice grain, but the work will also potentially provide information on genes responsible for transporting As and other contaminant metal(loid)s into the tissues of other edible plant organs. In addition to identifying the causal loci, synchrotron X-ray fluorescence microanalysis (SXRF) will be used to precisely localize and speciate As in plants, an innovative approach that has been used successfully to examine seed loading of As.

项目1：摘要 大米（Oryza sativa）是世界以上人口一半以上的主食，代表着重要的饮食来源 无机砷（AS），一种非阈值，1类人癌。必须采取策略来减少 谷物是开发的，并确定能够到达和积累的机制 米粒是这项工作的关键。该项目将阐明植物中AS稳态的遗传控制 启用不积聚的植物的发展。第一个特定目的检验了以下假设 大米的遗传多样性可以通过在已知的加入中绘制基因来利用较低的谷物作为水平。 谷物的内容各不相同。第二个目的询问转移的基因涉及哪些基因 谷物和第三个目标测试策略，根据信息限制射击/谷物的运动 从研究大米，拟南芥和累积的蕨类植物vittata的研究中获得。长期目标是 防止在水稻谷物的食用部分中积累，但工作也可能提供信息 关于负责运输AS和其他污染金属（LIE）S到其他可食用的组织的基因 植物器官。除了确定因果基因座，同步X射线荧光微分析（SXRF）之外 将被用来精确本地化和塑造植物，这是一种创新的方法 成功检查AS的种子负荷。