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The Genetic basis of Natural Ionomic Variation

自然离子组变异的遗传基础

基本信息

批准号：
8644802
负责人：
MARY LOU GUERINOT
金额：
$ 33.76万
依托单位：
DARTMOUTH COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-03-01 至 2017-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8644802
关键词：
Agriculture Alleles Attention Automobile Driving BTAF1 gene Biochemical Bioinformatics Biotechnology Chemistry Chromosome Mapping Cloning Collection Coupled DNA Developing Countries Disease Ecology Environment Epigenetic Process Food Future Generations Genes Genetic Genetic Models Genetic Polymorphism Genetic Variation Genomics Genotype Geographic Distribution Goals Haplotypes Health Human Investigation Iron Laboratories Link Macronutrients Nutrition Maintenance Maps Marines Mass Spectrum Analysis Methylation MicroRNAs Micronutrients Minerals Modeling Molecular Molecular Biology Molecular Genetics Mouse-ear Cress Nutrient Organism Phenotype Physiological Planet Mars Plants Plasma Population Population Genetics Process Property Quantitative Trait Loci RNA Interference Recombinants Regulation Research Resources Role Site Small Interfering RNA Solutions Source Spain Surface Sweden Testing Trace Elements Transact Transgenic Organisms Variant Zinc base climate change cost density design fitness follow-up gene function genetic linkage genome wide association study improved innovation insight interest international health organization life history nutrition positional cloning public health relevance research study response sample fixation soil sampling tool trait

项目摘要

DESCRIPTION (provided by applicant).Evolutionary forces are known to drive the fixation of locally adaptive genetic variation within populations, and such variation is thought to be involved in the differential response to disease and pharmacological agents observed in human populations. Natural variation is well documented in plants, and plants provide an excellent model for investigations into its genetic origins, maintenance and adaptive significance. We aim to take advantage of the natural genetic variation that occurs between populations of the genetic model plant Arabidopsis thaliana to identify gene functions that vary between these natural populations. Such a set of polymorphic genes and gene functions will provide the essential tools required to uncover the genetic mechanisms that drive the fixation of locally adaptive genetic variation within a population. To link natural genetic variation to function, we have applied high-throughput Inductively Coupled Plasma - Mass Spectroscopy (ICP-MS) based elemental-profiling platform, in combination with genome-wide association mapping and traditional linkage mapping to reveal loci that drive natural variation in the plant's elemental- profile or "ionome" including P, Ca, K, Mg (macronutrients); Cu, Fe, Zn, Mn, Co, Ni, Se, Mo, I (micronutrients of significance to plant and human health); Na, As, and Cd (minerals causing agricultural or environmental problems). Using a combination of laboratory and field-based experiments we propose to uncover the adaptive benefit and evolutionary significance of these naturally occurring polymorphic ionomic loci, along with their molecular function, and underlying causal genetic and epigenetic basis. This information will help determine the evolutionary processes involved in adaptation of organisms to local environments. Such insights will help in predicting the extent and limits of possible future adaptations of plants to changes in the surface chemistry of the Earth driven by a changing global climate, and will have applications to optimizing the mineral content of food crops for improved human health. After all, plants provide the major source of nutrition for a large portion of the world's population. Relevance Mineral nutrient deficiencies, such as iron and zinc, remain worldwide health concerns. Biofortification of food crops based on our research offers a sustainable, low-cost solution to this problem. Indeed, biofortification was recently ranked in the top 10 biotechnologies for improving health in developing countries.

描述（由申请人提供）：已知进化力量驱动群体内局部适应性遗传变异的固定，并且认为这种变异涉及在人类群体中观察到的对疾病和药理学试剂的差异反应。植物的自然变异是有据可查的，植物为研究其遗传起源、维持和适应意义提供了一个很好的模型。我们的目标是利用发生在遗传模式植物拟南芥种群之间的自然遗传变异，以确定这些自然种群之间不同的基因功能。这样一组多态性基因和基因功能将提供必要的工具，揭示遗传机制，驱动固定的局部适应性遗传变异的人口。为了将自然遗传变异与功能联系起来，我们应用了基于高通量电感耦合等离子体质谱（ICP-MS）的元素分析平台，结合全基因组关联作图和传统的连锁作图，以揭示驱动植物元素谱或“离子组”（包括P，Ca，K，Mg）中自然变异的基因座。（常量营养素）; Cu、Fe、Zn、Mn、Co、Ni、Se、Mo、I（对植物和人类健康具有重要意义的微量营养素）; Na、As和Cd（引起农业或环境问题的矿物质）。结合实验室和田间实验，我们建议揭示这些自然发生的多态性基因座的适应性益处和进化意义，沿着它们的分子功能，以及潜在的因果遗传和表观遗传基础。这些信息将有助于确定生物体适应当地环境的进化过程。这些见解将有助于预测植物未来可能适应全球气候变化所导致的地球表面化学变化的程度和限度，并将用于优化粮食作物的矿物质含量，以改善人类健康。毕竟，植物为世界上很大一部分人口提供了主要的营养来源。矿物质营养缺乏，如铁和锌，仍然是世界范围内的健康问题。基于我们研究的粮食作物生物强化为这一问题提供了可持续的低成本解决方案。事实上，生物强化最近被列为改善发展中国家健康的十大生物技术之一。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A coastal cline in sodium accumulation in Arabidopsis thaliana is driven by natural variation of the sodium transporter AtHKT1;1.

DOI：
10.1371/journal.pgen.1001193
发表时间：
2010-11-11
期刊：
PLoS genetics
影响因子：
4.5
作者：
Baxter I;Brazelton JN;Yu D;Huang YS;Lahner B;Yakubova E;Li Y;Bergelson J;Borevitz JO;Nordborg M;Vitek O;Salt DE
通讯作者：
Salt DE

Polyploids exhibit higher potassium uptake and salinity tolerance in Arabidopsis.

DOI：
10.1126/science.1240561
发表时间：
2013-08-09
期刊：
Science (New York, N.Y.)
影响因子：
0
作者：
Chao DY;Dilkes B;Luo H;Douglas A;Yakubova E;Lahner B;Salt DE
通讯作者：
Salt DE

Genome-wide association studies identify heavy metal ATPase3 as the primary determinant of natural variation in leaf cadmium in Arabidopsis thaliana.

DOI：
10.1371/journal.pgen.1002923
发表时间：
2012-09
期刊：
PLoS genetics
影响因子：
4.5
作者：
Chao DY;Silva A;Baxter I;Huang YS;Nordborg M;Danku J;Lahner B;Yakubova E;Salt DE
通讯作者：
Salt DE

Bulk segregant analysis using single nucleotide polymorphism microarrays.