project 1 - Renewal of the UMDNJ NeuroProteomics Core Facility

我们请求NINDS资助继续支持新泽西医学和牙科大学（UMDNJ）高级蛋白质组学研究中心（CAPR）内的神经蛋白质组学核心设施。在过去的资助期间，该核心成功地推进了神经科学研究和教育任务。它为NINDS研究人员提供了先进的蛋白质组学技术，并成为合作和新技术开发的中心。在此更新申请中，我们建议为NINDS资助的Pi提供最新的最先进的技术和升级的核心设备。这些扩展的功能包括应用生物系统4800串联质谱仪和其他最新的蛋白质组学设备，用于高通量蛋白质生物标志物的发现和验证。这些技术升级将实现的关键功能包括：1）更灵敏的识别和2）蛋白质磷酸化位点的定量 和其他翻译后修饰，这些系统将显着提高生产力的11个资格和其他神经科学研究项目（包括30多个NINDS研究人员）。蛋白质组学的成功应用需要先进的仪器和经验丰富的操作人员。NINDS核心赠款将提供精通样品制备，2D凝胶电泳，质谱和生物信息学的专门人员，以及与NINDS资格项目相关的仪器时间和用品，这一资源不会与现有设施重叠。为了确保核心的持续成功，UMDNJ为该核心提供了出色的机构承诺，包括新的实验室空间，仪器升级和支持人员的工资支持。由NINDS和其他CAPR调查人员组成的两个委员会将监督核心的财政和科学管理。用户俱乐部将为教育、培训、数据共享和协作提供一个重要论坛。正如核心过去的成功记录及其健全的科学和财务管理结构所证明的那样，这一改进后的核心将被很好地用于提高NINDS赞助的人类疾病研究的研究能力，包括多发性硬化症，帕金森氏症和其他神经退行性疾病。在NINDS资助的帮助下，神经科学研究人员将利用蛋白质组学技术来最大限度地发挥效益，以补充现有的研究方法，并促进新的研究方向和合作。

We are requesting NINDS funding for continued support of the NeuroProteomics Core Facility within the Center for Advanced Proteomics Research (CAPR) at the University of Medicine and Dentistry of New Jersey (UMDNJ). During the past funding period, this Core has successfully advanced both neuroscience research and educational missions. It has provided NINDS researchers access to advanced proteomics technologies, and has served as a hub for collaborations and new technology developments. In this renewal application, we are proposing to provide NINDS-funded Pi's with access to the latest state-of-the-art technologies with upgraded Core equipments. These expanded capabilities include an Applied Biosystems 4800 tandem mass spectrometer and other latest proteomics equipments for high throughput protein biomarker discovery and validation. Critical functions that will be fulfilled by these technology upgrades include: 1) more sensitive identification and 2) quantification of protein phosphorylation sites and other post-translational modifications, these systems will significantly enhance the productivity of the eleven qualifying and other neuroscience research projects (including over 30 NINDS investigators). Successful application of proteomics requires both advanced instrumentation and experienced operators. A NINDS core grant will provide dedicated personnel proficient in sample preparation, 2D gel electrophoresis, mass spectrometry and bioinformatics, as well as instrument time and supplies associated with NINDS qualifying projects, a resource that will not overlap with existing facilities. To ensure the continuous success of the Core, UMDNJ has provided excellent institutional commitments towards this Core including new laboratory space, instrumentation upgrade and salary support for the supporting personnel. Two committees, composed of both NINDS and other CAPR investigators, will oversee the Core's fiscal and scientific management. A User Club will provide an important forum for education, training, data sharing and collaborations. As demonstrated by the Core's past record of success and its sound scientific and financial management structure, this improved Core will be well utilized to enhance research capabilities for NINDS-sponsored studies of human diseases including multiple sclerosis, Parkinson's and other neurodegenerative diseases. With the benefit of a NINDS grant, neuroscience investigators will use proteomics technology to maximum benefits, to complement their existing research approaches and promote new research directions and collaborations.