Compact x-ray nanoscopy

X射线显微镜具有超高分辨率的潜力，因为它的衍射极限非常低，并且可以穿透厚样品。权衡是水性软组织样品中缺乏对比度。继我们在一个平行项目中开发了相衬成像技术宽带X射线源之后，我们正在从根本上重新评估在不产生高辐射剂量的情况下分辨软组织结构（如细胞器）的可能性。虽然有很有前途的技术相衬X射线显微镜，仔细考虑什么相衬和分辨率的基本限制可能在于什么是合适的应用程序在生物成像是必要的第一步之前，工程的努力开始。

X-ray microscopy has the potential for ultra-high resolution due to the very low diffraction limit, and penetration through thick samples. The trade-off is a lack of contrast in aqueous soft tissue samples. Following our development of phase-contrast imaging technologies broadband x-ray sources in a parallel project, we are re-assessing at a fundamental level the possibility of resolving soft tissue structures, such as cellular organelles, without incurring high radiation doses. Although there are promising technologies for phase-contrast x-ray microscopes, a careful consideration of what the fundamental limits of phase contrast and resolution may lie and what are the suitable applications in biological imaging is a necessary first step before the engineering effort begins.