X-ray micro tomography of pathology tissue samples

病理组织样本的 X 射线显微断层扫描

• 批准号: 10929149
• 负责人: HAN WEN
• 金额: $ 41.62万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10929149
• 关键词: Animal Model; Aorta; Atherosclerosis; Blood Vessels; Calibration; Cardiovascular Diseases; Collaborations; Deposition; Development; Dietary Supplementation; Fresh Tissue; Genetic; Histopathology; Image; International; Journals; Linoleic Acids; Lipids; Measurement; Methods; Microscope; Modeling; Molecular; Mus; National Heart, Lung, and Blood Institute; Oleic Acids; Pathology; Plasma; Process; Publishing; Resolution; Roentgen Rays; Sampling; Scanning; Science; Soybean Oil; Technology; Tissue Sample; Tissues; Unsaturated Fatty Acids; X ray microscopy; calcification; improved; microCT; mouse model; tomosynthesis

This is a continuing collaboration with Dr. Alan Remaley of NHLBI and Dr. Alexander Bocharov of the Clinincal Center. Using an x-ray microscopy technology we developed previously, we perform non-invasive measurements of the amount of tissue calcification in intact, fresh moue aorta samples for the various genetically modified moue models from our collaborators. As part of the technical development effort we published an online calibration method to improve the resolution of the scans 1. The measurements we made contributed to a study of the differential effect of soybean oil enriched with different types of unsaturated fatty acids on the development of atherosclerosis in a genetic mouse model 2. To further streamline the measurement process for a large number of samples, we are developing an inverted version of the x-ray microscope for better sample handling. 1. Bahar P, Nguyen D, Wang M, Mazilu D, Bennett EE, Wen H. Online Calibration of a Linear Micro Tomosynthesis Scanner. Journal of Imaging; 2022; 8: 292. 2. Yang Z-H, Nill K, Takechi-Haraya Y, Playford MP, Nguyen D, Yu Z-X, Pryor M, Tang J, Rojulpote KV, Mehta NN, Wen H, Remaley AT. Differential Effect of Dietary Supplementation with a Soybean Oil Enriched in Oleic Acid versus Linoleic Acid on Plasma Lipids and Atherosclerosis in LDLR-Deficient Mice. International Journal of Molecular Sciences; 2022; 23: 8385.

这是与NHLBI的Alan Remaley博士和临床中心的亚历山大Bocharov博士的持续合作。使用我们以前开发的x射线显微镜技术，我们对来自我们合作者的各种转基因小鼠模型的完整的新鲜小鼠主动脉样本中的组织钙化量进行了非侵入性测量。作为技术开发工作的一部分，我们发布了一种在线校准方法，以提高扫描分辨率1。我们所做的测量有助于研究富含不同类型不饱和脂肪酸的大豆油对遗传小鼠模型中动脉粥样硬化发展的差异效应2。为了进一步简化大量样品的测量过程，我们正在开发一种倒置的X射线显微镜，以更好地处理样品。 1. [10]杨文，王文，王文，王文，王文.线阵微型断层合成扫描仪的在线标定。影像学杂志; 2022; 8：292. 2. Yang Z-H，Nill K，Takechi-Haraya Y，Playford MP，Nguyen D，Yu Z-X，Pryor M，Tang J，Rojulpote KV，Mehta NN，Wen H，Remaley AT.膳食补充富含油酸和亚油酸的大豆油对低密度脂蛋白受体缺陷小鼠血脂和动脉粥样硬化的不同影响。国际分子科学杂志; 2022; 23：8385.