Tissue Procurement Core

组织采购核心

• 批准号: 8728160
• 负责人: IDHALIZ FLORES
• 金额: $ 16.07万
• 依托单位: PONCE SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8728160
• 关键词: Affect; Basic Science; Biocompatible Materials; Biological; Biological Markers; Blood; Cancer Center; Caribbean region; Clinic; Clinical; Collaborations; Collection; Confidentiality of Patient Information; Country; DNA; Data; Development; Diagnostic; Ensure; Environment; Epigenetic Process; Florida; Fostering; Fresh Tissue; Funding; Future; Genes; Genetic; Genomics; Hispanics; Histological Techniques; Human; Immunohistochemistry; Individual; Information Storage; Institution; Institutional Review Boards; Island; Latin America; Life; Liquid substance; Malignant Neoplasms; Medicine; Methods; Mexican Americans; MicroRNAs; Minority; Molecular; NCI Center for Cancer Research; Normal tissue morphology; Paraffin; Patients; Pharmacogenomics; Population; Positioning Attribute; Predisposing Factor; Preparation; Prevalence; Procedures; Process; Proteomics; Protocols documentation; Public Health; Puerto Rican; Puerto Rico; RNA; Research; Research Infrastructure; Research Personnel; Research Support; Resources; Sampling; Serum; Single Nucleotide Polymorphism; Solid; Specimen; Techniques; Technology; Tissue Banking; Tissue Banks; Tissue Microarray; Tissue Procurements; Tissues; Translations; Tumor Tissue; anticancer research; base; biobank; cancer Biomedical Informatics Grid; cancer health disparity; cancer type; high standard; human tissue; medical schools; novel; prognostic; protein expression; repository; research study; response; tool; trend; tumor

The purpose of the PC at Ponce School of Medicine (PSM) is to facilitate the collection, processing, and storage of well-annotated human biospecimens for use in investigator-initiated translational cancer research. Throughout the past five years a solid infrastructure was put in place to develop the first tumor tissue bank in Puerto Rico, capable of the optimal collection, processing, and distribution of biological materials derived from the island's Hispanic population. Through this unique resource, which follows the same standard operational procedures (SOPs) established at the Tissue Core at MCC (and which are approved by our institutions' IRB committees), fresh tissues and high quality RNA are already available to support research at both institutions focusing on the molecular aspects and biological determinants of cancer health disparities involving Puerto Rican Hispanics living in Puerto Rico and Hispanics living in Florida. This facility is a valuable resource of high public health significance given that 1) Hispanics represent 15% of the US population (the fastest growing minority population), 2) most cancer health-disparity studies in the US have been conducted with Mexican-American populations, 3) there are differences in cancer trends and unique aspects associated to cancer health disparities among the different Hispanic populations, and 4) there is a scarcity of basic science-oriented cancer studies involving Puerto Ricans. Availability of biospecimens (normal/tumor tissue, serum/DNA) from this population will support much needed research on cancer health disparities and facilitate answering questions on gene-environment-culture interactions involving Hispanics. We propose to expand and further develop the existing Partnership TPC to: 1) extend the collection of biological materials to include paraffin blocks [to support studies on protein expression, microRNA, and the preparation of Tissue Microarrays (TMA)], and of blood [to support future genetic, epigenetic and proteomic analyses]; and 2) to provide support in basic histological techniques, immunohistochemistry (IHC) methods, and advanced pathological analyses to investigators at PSM. TMAs of cancer types that predominantly affect the Hispanic population will be developed utilizing the MCC Tissue Core infrastructure. A DNA/serum repository of Puerto Rican patient samples will create a unique resource that will allow investigators to take advantage of high-throughput genomics- and proteomics-based technologies also available at MCC. These resources will support studies of factors (e.g., single nucleotide polymorphisms [SNPs], ancestry contribution) predisposing individuals to cancer development, the impact of SNPs on patient response to therapy (pharmacogenomics), and the discovery of diagnostic or prognostic molecular and proteomic signatures. With the rise of individualized/personalized medicine and biomarker discovery efforts, establishing a tissue/DNA/serum repository would be a valuable resource that can be used for furthering our understanding of the molecular factors that predispose or protect the Puerto Rican population to cancer. The genetic basis of known disparities in Puerto Rican Hispanics, which include different prevalence rates of certain types of cancer, can only be uncovered through access to these biospecimens. This partnership is uniquely positioned to fulfill this need. In fact, the U56-funded TPC already provides basic and clinical Partnership investigators with a centralized mechanism to collect, store, and process human tissues following standardized protocols. Because multi-parameter characterization of collected biospecimens will be required for the translation of experimental research findings to the clinic, a decision was made early on to utilize caBIG¿'s biorepository management tool, caTissue Suite, to collect and store associated data, which will facilitate the correlation of important clinical-pathological data with research results, while preserving patient confidentiality. PSM is the only cancer research center in Puerto Rico, the Caribbean, or Latin America to be connected to caBIG. Extending this practice to multiple tumor types, normal tissues, and fluid specimens would represent a leap to the next level of sophistication. Efforts are in place to ensure that tissue banking and information storage procedures are conducted using the highest standards in the field and are also attuned to other biobanking efforts across the country via participation in GMaP/BMaP (MCC and PSM are partners in Region 3). It is envisioned that the U54-funded, Hispanic-specific TPC will foster increased collaborations among the Partnership institutions and with other institutions, and will facilitate the application of novel molecular techniques to solving cancer health disparities involving Puerto Rican Hispanics (living on the island or in the mainland) and other Hispanics living in the US. In addition, this TPC constitutes a valuable asset for the recruitment of cancer researchers to the PSM-MCC Partnership.

Ponce医学院（PSM）的PC的目的是促进收集，处理和储存注释良好的人类生物标本，用于翻译启动的转化癌症研究。在过去的五年中，建立了坚实的基础设施，以开发波多黎各的第一个肿瘤组织库，能够最佳地收集、处理和分发来自该岛西班牙裔人口的生物材料。通过这一独特的资源，遵循MCC组织中心制定的相同标准操作程序（SOP）（并由我们机构的IRB委员会批准），新鲜组织和高质量RNA已经可以用于支持这两个机构的研究，重点是分子方面和癌症健康差异的生物学决定因素，涉及生活在波多黎各的波多黎各西班牙裔和生活在波多黎各的西班牙裔。佛罗里达。鉴于1）西班牙裔占美国人口的15%，该设施是一种具有高度公共卫生意义的宝贵资源（增长最快的少数民族人口），2）美国的大多数癌症健康差异研究都是在墨西哥裔美国人中进行的，3）不同西班牙裔人口之间的癌症趋势和与癌症健康差异相关的独特方面存在差异，4）缺乏涉及波多黎各人的基础科学导向的癌症研究。从这一人群的生物标本（正常/肿瘤组织，血清/DNA）的可用性将支持急需的癌症健康差异的研究，并有助于回答有关基因-环境-文化相互作用的问题，涉及西班牙裔。 我们建议扩大和进一步发展现有的TPC合作伙伴关系，以：1）扩大生物材料的收集，包括石蜡块[以支持蛋白质表达，microRNA和组织微阵列（TMA）的制备研究]和血液[以支持未来的遗传，表观遗传和蛋白质组学分析]; 2）为PSM的研究者提供基本组织学技术、免疫组化（IHC）方法和高级病理学分析方面的支持。 将利用MCC组织核心基础设施开发主要影响西班牙裔人群的癌症类型的TMA。波多黎各患者样本的DNA/血清储存库将创建一个独特的资源，使研究人员能够利用MCC也提供的高通量基因组学和蛋白质组学技术。这些资源将支持对各种因素的研究（例如，单核苷酸多态性[SNP]，祖先贡献）使个体易患癌症，SNP对患者治疗反应的影响（药物基因组学），以及诊断或预后分子和蛋白质组学特征的发现。随着个体化/个性化医学和生物标志物发现努力的兴起，建立组织/DNA/血清库将是一个宝贵的资源，可用于进一步了解使波多黎各人口易患或保护其患癌症的分子因素。波多黎各西班牙裔人中已知差异的遗传基础，包括某些类型癌症的不同流行率，只能通过获得这些生物标本来揭示。这种伙伴关系具有满足这一需求的独特优势。事实上，由U 56资助的TPC已经为基础和临床合作研究人员提供了一个集中的机制，以按照标准化协议收集、储存和处理人体组织。由于将实验研究结果转化为临床需要对收集的生物标本进行多参数表征，因此很早就决定利用caBIG的生物储存库管理工具caTissue Suite收集和存储相关数据，这将有助于将重要的临床病理数据与研究结果相关联，同时保护患者的机密性。PSM是波多黎各、加勒比海或拉丁美洲唯一一个与caBIG相连的癌症研究中心。将这一实践扩展到多种肿瘤类型、正常组织和液体标本将代表着向下一个复杂水平的飞跃。通过参与GMaP/BMaP（MCC和PSM是第3区的合作伙伴），努力确保组织库和信息存储程序使用该领域的最高标准进行，并与全国各地的其他生物库工作相协调。据设想，U 54资助的，西班牙裔特定的TPC将促进伙伴关系机构之间和与其他机构的合作，并将促进新的分子技术的应用，以解决涉及波多黎各西班牙裔（生活在岛上或大陆）和其他西班牙裔生活在美国的癌症健康差距。此外，该TPC是为PSM-MCC伙伴关系招募癌症研究人员的宝贵资产。