Genetic control of spermatogenesis: defining the role of SOX3 in spermatogonial progenitor cells

精子发生的遗传控制：定义 SOX3 在精原祖细胞中的作用

• 批准号: DP140103965
• 负责人: Prof Paul Thomas
• 金额: $ 27.25万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP140103965
• 关键词: Genetic; control; spermatogenesis; defining; role

The transcription factor (TF) SOX3 is a key regulator of neural stem/progenitor cells. Recently, this project has also shown that SOX3 is active in sperm progenitors (spermatogonia) and is required for spermatogenesis. Using our Sox3 KO mouse model and extensive expertise in spermatogonial cell culture, ChIP-seq technology and bioinformatics, this project will investigate crucial aspects of SOX3 function in the testes including stem versus progenitor cell activity and genome-wide target gene regulation. These studies will uncover the molecular and cellular mechanism by which SOX3 controls spermatogenesis and provide unique insight into how a single TF controls context-dependent differentiation in sperm versus brain progenitor cells.

转录因子（TF）SOX 3是神经干/祖细胞的关键调节因子。最近，该项目还表明，SOX 3在精子祖细胞（精原细胞）中具有活性，并且是精子发生所需的。利用我们的Sox 3 KO小鼠模型和精原细胞培养，ChIP-seq技术和生物信息学方面的广泛专业知识，该项目将研究SOX 3在睾丸中功能的关键方面，包括干细胞与祖细胞活性和全基因组靶基因调控。这些研究将揭示SOX 3控制精子发生的分子和细胞机制，并为单个TF如何控制精子与脑祖细胞的上下文依赖性分化提供独特的见解。