Dynorphins and Kappa Opioid Receptors in the Stress Response

描述（由申请人提供）：这是第一次更新申请，要求高级科学家研究和导师奖（K 05）的第二个5年期的支持。目前的奖项对我作为科学领导者和导师的职业发展非常重要，使我能够专注于这些责任。在此期间，我的科学领导工作包括UW药物成瘾研究中心的方向，开发核心行为，成像和蛋白质组学研究设施，促进科学合作，促进社区外展。我的校外领导包括组织定于2011年7月举行的关于“Kappa阿片类药物在疼痛和成瘾方面的治疗潜力”的科学会议，并在国际麻醉品研究会议（INRC）执行委员会任职。目前的K 05奖使我能够专注于研究进展，并在过去4年多的时间里发表了28篇同行评审的出版物，这些出版物采用了新方法，产生了新的见解，并提出了新的假设。目前的资助期间支持了我的导师活动的扩展，包括NIDA T32“滥用药物分子药理学培训”的更新和ARRA-NIDA P30“药物滥用功能蛋白质组学方法的新教师搜索”的奖项，这两个都是我指导的。K 05，P30和T32奖项鼓励我积极参与“生物医学研究诚信培训计划”，并积极指导研究生和本科生，博士后研究员和新教师。K 05的下一个支持期将建立在这个基础上，保护我的时间不受额外的服务教学和行政责任的影响，并使我能够致力于3个研究目标：首先，检验阿片受体激活c-Jun激酶提供GPCR信号调节的新机制的假设。第二，p38 MAPK的κ阿片受体激活启动了负责应激的烦躁和焦虑效应的信号传导事件。第三，在下一个支持阶段，K 05将使我的实验室能够在蛋白质分析化学技术方面获得优势，这些技术是定义阿片受体信号复合物组分所必需的。在目前的资助期内，我已经指导了3名博士后研究员谁去了教师职位（Bruchas，Macey & Gendron）; 2名初级教师谁建立了成功的研究项目，并赢得了晋升（菲利普斯和斯特拉）;和3名研究生谁赢得了博士学位，并提前到博士后奖学金职位。在下一个资助期内，我希望积极促进两名新的终身助理教授（Zwiefel和Ong），5名研究生和3名博士后研究员的专业发展。

DESCRIPTION (provided by applicant): This is a first renewal application requesting a second 5-yr period of support of a Senior Scientist Research and Mentorship Award (K05). The current award has been extremely important for my professional development as a scientific leader and mentor by allowing me to focus my effort on those responsibilities. My scientific leadership efforts during this period include direction of a UW Center for Drug Addiction Research, developing core behavioral, imaging and proteomic research facilities, fostering scientific collaborations, and promoting community outreach. My extramural leadership includes organization of a scientific conference scheduled in July 2011 on the "Therapeutic Potential of Kappa Opioids in Pain and Addiction" and service on the executive committees of the International Narcotics Research Conference (INRC). The current K05 award has enabled me to focus my effort on research progress and has resulted in 28 peer-reviewed publications during the last 4+ years that incorporated new approaches, generated new insights, and developed novel hypotheses. The current funding period has supported an expansion of my mentorship activities including the renewal of a NIDA T32 "Training in Molecular Pharmacology of Abused Drugs" and the award of an ARRA-NIDA P30 "New Faculty Search For Functional Proteomic Approaches To Drug Abuse," both of which I direct. The K05, P30 and T32 awards have encouraged my active participation in a "Biomedical Research Integrity Training Program" and enabled active mentoring of graduate and undergraduate students, postdoctoral fellows, and new faculty. The next period of K05 support would build on this foundation by protecting my time from extra service-teaching and administrative responsibilities and allowing me to devote my efforts towards 3 research goals: First, to test the hypothesis that opioid receptor activation of c-Jun Kinase provides a novel mechanism of GPCR signal regulation. Second, that kappa opioid receptor activation of p38 MAPK initiates signaling events responsible for the dysphoric and anxiogenic effects of stress. And third, in the next period of support, the K05 would enable my lab to gain strength in protein analytical chemistry techniques necessary to define components of the opioid receptor signaling complex. In the current funding period, I have mentored 3 postdoctoral fellows who have gone on to faculty positions (Bruchas, Macey & Gendron); 2 junior faculty members who have built successful research programs and earned promotion (Phillips and Stella); and 3 graduate students who earned their doctorates and advanced to postdoctoral fellowship positions. In the next funding period, I expect to actively promote the professional development of two new tenure-track assistant professors (Zwiefel and Ong), 5 graduate students & 3 postdoctoral fellows currently working in the lab.