High Resolution Imaging Shared Facility

高分辨率成像共享设施

• 批准号: 9895647
• 负责人: Alexa Lynn Mattheyses
• 金额: $ 17.03万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9895647
• 关键词: Anesthesia procedures; Animal Disease Models; Animals; Biological Markers; Body Temperature; Cancer Center Support Grant; Cells; Complex; Comprehensive Cancer Center; Computer software; Consultations; Cost Sharing; Data Analyses; Detection; Development; Disease Progression; Education and Outreach; Electron Microscope; Ensure; Equipment; Ethics; Event; Fluorescence Resonance Energy Transfer; Genetic; Grant; Image; Image Analysis; Image Cytometry; Imaging technology; In Vitro; Investigation; Laboratory Personnel; Laser Scanning Confocal Microscopy; Laser Scanning Microscopy; Lasers; Maintenance; Malignant Neoplasms; Manuscripts; Methodology; Microscopic; Microscopy; Mission; Molecular Analysis; Preparation; Research Personnel; Research Support; Resistance; Resolution; Resource Sharing; Resources; Respiration; Role; Scanning; Scanning Electron Microscopy; Services; Specialist; Specimen; Speed; Students; System; Systems Analysis; Technical Expertise; Testing; Therapeutic; Time; Tissue imaging; Tissues; Training; Training and Education; Vision; Work; anticancer research; biological systems; cancer therapy; cellular imaging; design; digital; digital imaging; exosome; experience; experimental study; fluorescence lifetime imaging; gene interaction; gene product; high resolution imaging; image processing; imaging capabilities; imaging study; in vivo; instrumentation; intravital imaging; laboratory experience; lectures; live cell imaging; member; molecular imaging; nanoparticle; programs; student training; tool; translational study; transmission process; vibration

IMAGING GROUP HIGH RESOLUTION IMAGING SHARED FACILITY (HRISF) ABSTRACT The detection and imaging of molecules in biological systems is a vitally important tool in cancer research. As the genetic basis for cancers are identified, the subsequent objectives are to identify the localization, functions, and interactions of gene products and to determine their role in the initiation and progression of disease. These objectives require highly sophisticated methodologies for the analysis of molecular events in vitro, in live cells, and in animal models of disease. Therefore, the High Resolution Imaging Shared Facility (HRISF) provides Comprehensive Cancer Center investigators with access to complex instrumentation required for the detailed microscopic analysis of cells and tissues and the expertise to use it productively. The imaging resources available include: 1) digital imaging; 2) Ca2+ imaging; 3) a FRET system; 4) a Zeiss LSM 710 equipped for fluorescence lifetime imaging (FLIM); 5) a Nikon A1R high speed confocal system optimized for live cell and tissue imaging; and 6) a Nikon A1R Multiphoton system optimized for intravital imaging with a Coherent Chameleon Vision II ultrafast laser. In addition, the Nikon A1R Multiphoton system is mounted on a vibration isolation table and has all of the equipment required for long-term animal anesthesia, respiration, and body temperature maintenance. Recent additions to the shared resource include an ImageStream X Imaging cytometer which allows high throughput imaging - over 5000 cells/second can be imaged with up to 12 images/cell. We also obtained a Nanosight NS300 which allows characterization of exosomes and nanoparticles. For ultrastructural studies the HRISF offers an FEI Quanta 650 FEG Environmental Scanning EM and a Tecnai T12 Spirit TWIN transmission electron microscope. Cancer-related research supported by the HRISF includes translational studies that range from fundamental studies of acquired resistance to frontline cancer therapeutics. The HRISF served over 88 CCC investigators during the current CCSG cycle and their use accounted for 49.5% of the total facility usage during this same period. At the same time, support from the CCSG accounted for 23% of the total annual operating costs of the shared resource.

成像集团 高分辨率成像共享因子（HRISF） 摘要 生物系统中分子的检测和成像是癌症研究中至关重要的工具。作为 确定癌症的遗传基础，随后的目标是确定癌症的定位，功能， 和基因产物的相互作用，并确定它们在疾病的发生和发展中的作用。 这些目标需要高度复杂的方法学来分析体外、活体和体内的分子事件。 细胞和疾病的动物模型。高分辨率成像共享设施（HRISF） 为综合癌症中心的研究人员提供了使用复杂仪器的机会， 对细胞和组织进行详细的显微镜分析，以及有效使用它的专业知识。成像 可用的资源包括：1）数字成像; 2）Ca 2+成像; 3）FRET系统; 4）Zeiss LSM 710 配备荧光寿命成像（FLIM）; 5）Nikon A1 R高速共聚焦系统， 活细胞和组织成像;以及6）Nikon A1 R Multiphoton系统，其优化用于活体成像， 相干变色龙视觉II超快激光。此外，尼康A1 R多光子系统安装在一个 振动隔离台，并具有长期动物麻醉，呼吸， 维持体温最近增加的共享资源包括ImageStream X成像 允许高通量成像的细胞仪-超过5000个细胞/秒可以成像， images/cell.我们还获得了Nanosight NS 300，其允许表征外泌体， 纳米粒子对于超微结构研究，HRISF提供FEI Quanta 650 FEG环境扫描 EM和Tecnai T12 Spirit TWIN透射电子显微镜。癌症相关研究由 HRISF包括转化研究，从获得性耐药性的基础研究到前线研究， 癌症治疗学HRISF在CCSG当前周期内为88名CCC调查员提供服务， 使用量占同期设施总使用量的49.5%。与此同时， CCSG占共享资源年度总运营成本的23%。