DNA nanotechnology for controlled antigen presentation to T cells

DNA 纳米技术可控制抗原呈递至 T 细胞

• 批准号: DP170102727
• 负责人: Prof Katharina Gaus
• 金额: $ 33.97万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-01-01 至 2020-03-01
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP170102727
• 关键词: DNA; nanotechnology; controlled; antigen; presentation

The project aims to present individual antigens to T cells and to image T cell receptor signalling with single molecule microscopy. Combining DNA origami nanotechnology with single molecule imaging should reveal the sensitivity of T cell signalling. A DNA force sensor will determine whether mechanical forces contribute to antigen discrimination. The project will use the nanotechnology strategy to identify antigen-specific T cells in tissue. The project is expected to advance understanding of T cell biology, and contribute to DNA nanotechnology and super-resolution microscopy whilst providing fundamental insights into antigen recognition by T cells and ultimately derive clinically relevant practical applications.

该项目旨在将单个抗原呈递给T细胞，并利用单分子显微镜成像T细胞受体信号。结合DNA折纸纳米技术与单分子成像应该揭示T细胞信号传导的敏感性。DNA力传感器将确定机械力是否有助于抗原识别。该项目将使用纳米技术策略来识别组织中的抗原特异性T细胞。该项目预计将促进对T细胞生物学的理解，并有助于DNA纳米技术和超分辨率显微镜，同时提供T细胞识别抗原的基本见解，并最终获得临床相关的实际应用。