Confocal and single molecule microscopes for systems microscopy

用于系统显微镜的共焦和单分子显微镜

• 批准号: LE180100157
• 负责人: Prof Katharina Gaus
• 金额: $ 40.01万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2018
• 资助国家: 澳大利亚
• 起止时间: 2018-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE180100157
• 关键词: Confocal; single; molecule; microscopes; systems

This project aims to establish Australia’s first system microscopy facility with dedicated live-cell confocal and single-molecule fluorescence microscopes. In systems microscopy, the imaging workflow is automated so that large and unbiased data sets of the spatiotemporal organisation of molecules and cells can be generated. Combined with statistical and bioinformatics analyses, image-derived data provides system-wide information that is not easily obtainable with other approaches. The project will enable Australian researchers to image and analyse the full complexity of biological systems, potentially transforming cell biology, drug development and understanding the molecular basis of disease. It will also demonstrate how the capacity of microscopy facilities can be enhanced and bias in imaging data reduced by automating data acquisition and mining of image-based data.

该项目旨在建立澳大利亚第一个具有专用活细胞共聚焦和单分子荧光显微镜的系统显微镜设施。在系统显微镜中，成像工作流程是自动化的，因此可以生成分子和细胞时空组织的大型无偏数据集。结合统计和生物信息学分析，图像衍生数据提供了用其他方法不易获得的全系统信息。该项目将使澳大利亚研究人员能够对生物系统的全部复杂性进行成像和分析，从而可能改变细胞生物学、药物开发和理解疾病的分子基础。它还将展示如何通过自动化数据采集和基于图像的数据挖掘来增强显微镜设施的能力并减少成像数据的偏差。