High-resolution reflective microscope

高分辨率反射显微镜

• 批准号: 10546300
• 负责人: PETRA E WILDER-SMITH
• 金额: $ 25.92万
• 依托单位: LIGHT RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10546300
• 关键词: Address; Adopted; Arizona; Biomedical Research; Cell physiology; Cells; Characteristics; Chemicals; Collection; Data; Data Collection; Development; Diamond; Goals; Image; Imaging Techniques; Imaging technology; Knowledge; Length; Licensing; Light; Measures; Mechanics; Methods; Microscope; Microscopy; Multimodal Imaging; Optics; Outcome; Performance; Phase; Process; Production; Property; Research; Research Personnel; Resolution; Series; Small Business Innovation Research Grant; Speed; Subcellular structure; Surface; System; Techniques; Technology; Testing; Time; Tissues; Tube; Universities; Work; absorption; biological research; contrast imaging; cost; data acquisition; design; high resolution imaging; imaging modality; imaging system; innovation; lens; microscopic imaging; multimodal data; multimodality; prevent; prototype; tool; ultraviolet

The goal of this SBIR project is to develop and commercialize a fundamentally new type of all-reflective microscope (ARM) without any obscuration for full spectrum applications from ultraviolet (UV) to infrared (IR). In Phase I stage, Light Research Inc (LRI) will demonstrate ARM by designing, prototyping, and characterizing a high numerical aperture (NA) reflective objective and tubing lens. Current refractive microscope has a number of limitations due to the material dispersion and absorption: chromatic aberrations, throughput, group delay dispersion (GDD), and narrow working spectral range. While successfully addressing the limitations on chromatic aberration and group delay dispersion, the traditional solution - on-axis reflective objective - still has some other major issues due to the central obscuration, preventing it from being widely adopted in biological research: low throughput, low contrast, and low image quality. To address all limitations in the current state-of-the-art refractive and reflective microscope, a fundamentally new and true ARM without obscuration will be developed. The key innovation of this ARM is the off-axis configuration with freeform surfaces. Off-axis, non-obscuration objective with freeform surfaces will address all issues related to traditional reflective objective, and has the following unique key properties: high image contrast, high image quality, high light efficiency, high throughput, zero chromatic aberration, and low GDD. LRI will license the snap- on assembling technology from University of Arizona (UA) to develop and commercialize the alignment-free off- axis, non-obscuration reflective objective. The goal of the Phase I project will be achieved through two aims: (1) design ARM, and (2) prototype and characterize ARM. With the gained knowledge and successful demonstration of ARM in Phase I project, in Phase II LRI will develop and commercialize a series of reflective objectives with different NAs and tube lenses with different focal lengths. With the unique capability of high-performance imaging from UV to IR without any obscuration, the true ARM will not only simplify the configurations of the current multimodal and broadband imaging systems, but also enable investigators to advance the biological and biomedical research through acquiring new multimodal and broadband data with higher throughput and speed in full spectrum, and developing new imaging techniques not possible with current microscope platform.

这个SBIR项目的目标是开发和商业化一种全新的全反射型 这是一种无任何遮蔽的全光谱显微镜（ARM），适用于从紫外线（UV）到红外线（IR）的全光谱应用。在 在第一阶段，Light Research Inc（LRI）将通过设计、原型制作和表征一个 高数值孔径（NA）反射物镜和管状透镜。 由于材料的色散和吸收，当前的折射显微镜具有许多限制： 色差、吞吐量、群延迟色散（GDD）和窄工作光谱范围。而 成功地解决了色差和群延迟色散的限制，传统的 解决方案-轴上反射物镜-仍然具有一些其他的主要问题，由于中心遮蔽， 它在生物研究中被广泛采用：低通量、低对比度和低图像质量。 为了解决当前最先进的折射和反射显微镜中的所有限制， 真正的无遮蔽反辐射导弹将被研制出来。这款ARM的关键创新在于离轴配置 自由曲面。具有自由曲面的离轴非遮蔽物镜将解决所有相关问题 与传统的反射物镜相比，具有以下独特的关键性能：高图像对比度，高图像分辨率， 高质量、高光效、高吞吐量、零色差和低GDD。LRI将授权管理单元- 亚利桑那大学（UA）的组装技术，以开发和商业化无污染的关闭， 轴，非遮蔽反射物镜。 第一阶段项目的目标将通过两个目标实现：（1）设计ARM，（2）原型和 随着在第一阶段项目中获得的知识和对ARM的成功演示， II LRI将开发并商业化一系列具有不同NA和管透镜的反射物镜， 不同的焦距。 凭借从紫外到红外的高性能成像的独特能力，真正的ARM将 不仅简化了当前多模态和宽带成像系统的配置， 研究人员通过获得新的多模态和 宽带数据，具有更高的吞吐量和速度，在全光谱，并开发新的成像技术， 目前的显微镜平台。