Heterologous expression of cyanobacterial compounds of analytical and therapeutic value

具有分析和治疗价值的蓝藻化合物的异源表达

• 批准号: LP140100642
• 负责人: Prof Brett Neilan
• 金额: $ 28.3万
• 依托单位: The University of Newcastle
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-01-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP140100642
• 关键词: Heterologous; expression; cyanobacterial; compounds; analytical

The project team has previously discovered the genetic basis for toxin production in cyanobacteria and algae. This project aims to define the biochemical pathways of the unique biosynthetic enzymes involved in alkaloid and cyclic peptide toxin production in these microorganisms. Via the development of large-gene cloning strategies, the toxins will be produced in a stable, sustainable and secure fermentation system. These compounds will be critical for standardising toxin detection methods in water supplies globally and for assessing their bioactivities in humans and other animals. Research students and the industry partner will also be trained to apply these novel biotechnologies for the production of other drug-like molecules.

该项目团队此前已经发现了蓝藻和藻类产生毒素的遗传基础。该项目旨在确定这些微生物中参与生物碱和环肽毒素产生的独特生物合成酶的生化途径。通过开发大基因克隆策略，毒素将在稳定、可持续和安全的发酵系统中生产。这些化合物将对标准化全球供水中的毒素检测方法以及评估它们在人类和其他动物身上的生物活性至关重要。研究学生和行业合作伙伴还将接受培训，将这些新的生物技术应用于生产其他类似药物的分子。