West African Emerging Infectious Disease Research Center (WA-EIDRC)

先前的研究表明，在大流行之前，塞拉利昂人更频繁地 比美国人暴露于冠状病毒的表位与严重急性 呼吸道综合征冠状病毒2型（SARS-CoV-2）、SARS-CoV和中东呼吸道 综合征冠状病毒（MERS-CoV）。既往暴露于冠状病毒，可诱导交叉保护 免疫力可能有助于大幅降低塞拉利昂的COVID-19病例和死亡率 塞拉利昂与美国相比。这种补充将（1）定义体液免疫 塞拉利昂人的反应，可以防止感染SARS-CoV-2， 大流行前单克隆抗体分离和鉴定，（2）开发诊断 新型冠状病毒的测试和（3）确定交叉中和的保护性反应 在非人灵长类动物COVID-19模型中的单克隆抗体。

Prior studies have shown that prior to the pandemic, Sierra Leoneans had more frequent exposures than Americans to coronaviruses with epitopes that cross-react with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), SARS-CoV, and Middle Eastern respiratory syndrome coronavirus (MERS-CoV). Prior exposure to coronaviruses that induce cross-protective immunity may contribute to the greatly reduced incidence of COVID-19 cases and deaths in Sierra Leone compared to the United States. This supplement will (1) define the humoral immune response of Sierra Leoneans that may protect against infection with SARS-CoV-2 through isolation and characterization of pre-pandemic monoclonal antibodies, (2) develop diagnostic tests for novel coronaviruses and (3) determine the protective responses of cross neutralizing monoclonal antibodies in a nonhuman primate COVID-19 model.