FLUCTUATION CORRELATION SPECTROSCOPY FOR NANO GOLD PARTICLE ON CELL MEMEBRANES

细胞膜上纳米金颗粒的涨落相关光谱

• 批准号: 7600939
• 负责人: ENRICO GRATTON
• 金额: $ 9.46万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7600939
• 关键词: Behavior; Cells; Computer Retrieval of Information on Scientific Projects Database; Diffuse; Fluorescence; Fluorescent Probes; Funding; Gold; Gold Colloid; Grant; Institution; Manufacturer Name; Research; Research Personnel; Resources; Source; Specific qualifier value; Spectrum Analysis; Time; United States National Institutes of Health; detector; nanoGold; nanoparticle; particle; research study; size

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We observed the intensity fluctuation due to plasmon resonance of diffusing spherical, colloidal gold nano-particles and compared it with the fluctuation obtained with common fluorescent probes. The auto correlation curves obtained form the fluctuation correlation experiment showed unexpected behavior of the correlation times and PCH distribution for the plasmon resonant gold particles than for the fluorescent probes, assuming a nominal size for the gold particle as specified by the manufacturer. We are systematically investigating the origin of this discrepancy. One possibility is that the focus of the fluorescence and of the scattering volume observed is not identical. Another effect could be related to deformation of the PSF due to the very high instantaneous intensity of the gold particle that transiently saturate the detector. The state of aggregation of the gold particles could also be responsible for part of the discrepancy.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们观察到由于等离子体共振扩散的球形，胶体金纳米颗粒的强度波动，并与普通荧光探针获得的波动进行比较。 从波动相关性实验获得的自相关曲线显示了等离子体共振金颗粒的相关时间和PCH分布的出乎意料的行为，而不是荧光探针，假设制造商指定的金颗粒的标称尺寸。 我们正在系统地调查这种差异的根源。 一种可能性是观察到的荧光和散射体积的焦点不相同。 另一个效应可能与PSF的变形有关，这是由于金粒子的非常高的瞬时强度使检测器瞬时饱和。金颗粒的聚集状态也可能是造成这种差异的部分原因。