IMPROVED MASS MEASUREMENT IN FTMS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 准确的质量测量能力是傅里叶变换质谱仪(FTMS)的一个重要属性。与质谱学中的其他仪器方法不同，FTMS仍然提供了提高质量测量精度(MMA)的重要机会，使其成为一个研究领域。尽管FTMS可以测量到九位数的离子频率，但频率到m/z的转换是困难的，因为频率取决于离子陷阱中的电场和空间电荷。我们正在研究填补电场(电气补偿)和修正空间电荷效应的方法。我们还对质量校准与离子运动和空间电荷效应的基本原理之间的关系感兴趣，我们希望改进基本的校准程序，特别是那些校正空间电荷效应的程序。基质辅助激光解吸电离(MALDI)和电喷雾电离(ESI)的出现为FTMS用于分析包括蛋白质、寡核苷酸(ODN)、寡糖和合成聚合物在内的高性能生物聚合物打开了大门。我们将在这些领域追求准确的质量测量，并寻求一些切实可行的方法来改进MMA。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The capability for accurate mass measurements is an important attribute of Fourier transform mass spectrometry (FTMS). Unlike other instrumental methods in mass spectrometry, FTMS still offers significant opportunities to improve mass measurement accuracy (MMA), making it an area of research. Although FTMS can measure ion frequencies to nine significant figures, the conversion of frequency to m/z is difficult because frequency depends on the electric field and the space charge in the ion trap. We are working on methods to shim the electric field (electrical compensation) and to correct for space charge effects. We are also interested in the relation of mass calibration and the fundamentals of ion motion and space-charge effects, and we wish to to improve the basic calibration procedure particularly those that correct for effects of space charge. The advent of matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) have opened the door for FTMS to be used for analyzing at high performance biopolymers, including proteins, oligodeoxynucleotides (ODNs), oligosaccharides, and synthetic polymers. We will pursue accurate mass measurement in these areas and some practical ways to improve MMA.