IMPROVED MASS MEASUREMENT IN FTMS

改进 FTMS 中的质量测量

• 批准号: 8361343
• 负责人: MICHAEL L GROSS
• 金额: $ 0.94万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361343
• 关键词: Area; Biopolymers; Calibration; Charge; Electrospray Ionization; Financial compensation; Fourier Transform; Frequencies; Funding; Grant; Ions; Mass Spectrum Analysis; Measurement; Measures; Methods; Motion; National Center for Research Resources; Oligosaccharides; Performance; Polymers; Principal Investigator; Procedures; Proteins; Research; Research Infrastructure; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; United States National Institutes of Health; Work; biomedical resource; cost; electric field; improved; interest

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The capability for accurate mass measurements is an important attribute of Fourier transform mass spectrometry (FTMS). Unlike other instrumental methods in mass spectrometry, FTMS still offers significant opportunities to improve mass measurement accuracy (MMA), making it an area of research. Although FTMS can measure ion frequencies to nine significant figures, the conversion of frequency to m/z is difficult because frequency depends on the electric field and the space charge in the ion trap. We are working on methods to shim the electric field (electrical compensation) and to correct for space charge effects. We are also interested in the relation of mass calibration and the fundamentals of ion motion and space-charge effects, and we wish to to improve the basic calibration procedure particularly those that correct for effects of space charge. The advent of matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) have opened the door for FTMS to be used for analyzing at high performance biopolymers, including proteins, oligodeoxynucleotides (ODNs), oligosaccharides, and synthetic polymers. We will pursue accurate mass measurement in these areas and some practical ways to improve MMA.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 精确质量测量的能力是傅里叶变换质谱 (FTMS) 的一个重要属性。 与质谱分析中的其他仪器方法不同，FTMS 仍然提供了提高质量测量精度 (MMA) 的重要机会，使其成为一个研究领域。 尽管 FTMS 可以测量精确到九个有效数字的离子频率，但将频率转换为 m/z 很困难，因为频率取决于电场和离子阱中的空间电荷。 我们正在研究匀场电场（电补偿）和校正空间电荷效应的方法。 我们还对质量校准与离子运动和空间电荷效应的基本原理之间的关系感兴趣，并且我们希望改进基本校准程序，特别是那些校正空间电荷效应的校准程序。 基质辅助激光解吸电离 (MALDI) 和电喷雾电离 (ESI) 的出现为 FTMS 用于分析高性能生物聚合物打开了大门，包括蛋白质、寡脱氧核苷酸 (ODN)、寡糖和合成聚合物。 我们将在这些领域追求精确的质量测量以及改进 MMA 的一些实用方法。